Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications
Composition Highly cross-linked polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Working pH range 3–11
Dynamic Binding Capacity* 30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical Stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C

*   10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Binding Capacity
Protein and Plasmid Purification
UNOSphere™ Rapid S media UNOSphere Rapid S Strong cation exchange SO3- Human IgG: 60 mg/ml*
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite
Hydroxyapatite
Fluoropatite
(Ca5(PO4)3OH)2
Ca10(PO4)6F2
Human IgG; 25-60 mg/ml**
Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media UNOSphere Rapid S Strong cation exchange SO3- Human IgG: 60 mg/ml*
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite
Hydroxyapatite
Fluoropatite
(Ca5(PO4)3OH)2
Ca10(PO4)6F2
Human IgG; 25–60 mg/ml**
Lysozyme 15–21 mg/g
Antibody Purification
CFT Media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
UNOsphere SUPrA™ media UNOsphere SUPrA Affinity Protein A  
Monoclonal Antibody Purification
mAb purification kit UNOsphere SUPrA
CHT™ Type 1
UNOsphere Q
Affinity
Hydroxyapatite
Strong anion exchange
Protein A
(Ca5(PO4)3OH)2
-N(CH3)3*
 
Human IgG: 25–60 mg/ml**
BSA* 180 mg/ml

* Flow rate = 150 cm/hr.
** Flow rate = 300 cm/hr.

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml–40 mm length x 5.6 mm inner diameter
5 ml–40 mm length x 12.6 mm inner diameter
10 ml–80 mm length x 12.6 mm inner diameter**
50 ml–80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material of animal origin.

Bio-Scale Mini cartridges have a polypropylene double-wall construction* that provides extra strength and allows reliable runs up to 45 psi with the aqueous buffers most commonly used for protein separations. Bio-Scale Mini cartridges are ideal for low-pressure chromatography applications. Luer fittings make connections simple; adapters are available to allow connection to all types of chromatography systems.

Bio-Scale Mini UNOsphere SUPrA cartridges are available in 1 ml and 5 ml sizes. These cartridges are prepacked with UNOsphere SUPrA affinity media and are ideal for method scouting and small-scale purifications.

* U.S. Patent 7,208,087.

Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 1ml

732-4400
Pkg of 5, 1 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $189.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 1 x 5 ml

732-4401
5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $133.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 5 ml

732-4402
Pkg of 5, 5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $461.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml

732-4200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $143.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml

732-4201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $620.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml

732-4202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $527.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
5729 UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5728 Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A Click to download [ Add to Cart (Free) ]
10014997 Instruction Manual, Bio-Scale Mini UNOsphere SUPrA cartridges, 1 and 5 ml, Rev A Click to download
10014430 Instruction Manual, UNOsphere SUPrA Affinity Chromatography Media, Rev A Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download