Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges

Bio-Scale Mini UNOsphere Rapid S Cartridges


Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue


0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A UNOsphere SUPrA™ Affinity Recombinant protein A 30 mg/ml human IgG 732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT Ceramic
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
5 x 5
5 x 10
1 x 50
5 x 50
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

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Prepacked Bio-Scale™ Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with aqueous buffers most commonly used for protein separation. The cartridges are prepacked with media for ion exchange, affinity, and hydroxyapatite and fluoroapatite chromatography. The cartridges are convenient, ready to use, and disposable, and the simple luer connections connect easily to any chromatography system (see bulletin 5326 under the Documents tab). Bio-Rad now offers UNOsphere™ Rapid S, UNOsphere SUPrA™, and CFT™ ceramic fluoroapatite media prepacked in Bio-Scale Mini cartridges and two kits: one for purification using apatite (CFT ceramic fluoroapatite and CHT™ ceramic hydroxyapatite) and one for monoclonal antibody purification.

UNOsphere Rapid S strong cation exchange media have enhanced chemistries to overcome the pH shift that occurs with conductivity transitions. The support quickly adjusts to changes in conductivity with minimal pH excursion. No pH shifting provides a new level of economy due to shorter equilibration times, resulting in faster separations and lower consumption of buffer solutions. The media have been optimized to deliver speed and throughput in capture and intermediate purification steps. UNOsphere Rapid S media combine high binding capacity, fast mass transfer, excellent flow characteristics, and robust physical properties to make it an exceptional choice for purification.

  • Available in 1 and 5 ml cartridges
  • Excellent scalability
  • Stable in 1 M NaOH
  • High binding capacity
  • High linear flow rates at low backpressures
  • Rapid regeneration
  • Fully supported for regulatory submissions
  • Engineered for reproducible results

* U.S. patent 7,208,087 B2.


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(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 5 x 1 ml

Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 1 x 5 ml

Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 5 x 5 ml

Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
10005454 Instruction Manual, Bio-Scale™ Mini UNOsphere™ Q, S and Rapid S Cartridges, 1 and 5 ml, Rev E Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download

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