Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges

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Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
Histidine-tagged protein
780–0811 780–0812
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini Macro-Prep High Q, High S, and DEAE cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with either Macro-Prep High Q, Macro-Prep High S, or Macro-Prep DEAE support:

  • High Q — strong anion exchange; chemical form –N+(CH3)3, protein capacity ≥37 mg/ml BSA
  • High S — strong cation exchange; chemical form –SO3-, the ion-moderated partition chromatography technique, protein capacity ≥49 mg/ml human IgG
  • DEAE — weak anion exchange; chemical form –HN+(C2H5)2, protein capacity >35 mg/ml protein

Macro-Prep ion exchange media meet the demands of analytical applications. These rigid, macroporous hydrophilic media provide exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

* U.S.patent 7,208,087.

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  
Bio-Scale Mini Macro-Prep High Q Cartridges

732-4120
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $194.00
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Bio-Scale Mini Macro-Prep High Q Cartridge

732-4122
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $76.00
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Bio-Scale Mini Macro-Prep High Q Cartridges

732-4124
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $271.00
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Bio-Scale Mini Macro-Prep High S Cartridges

732-4130
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

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Bio-Scale Mini Macro-Prep High S Cartridge

732-4132
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $76.00
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Bio-Scale Mini Macro-Prep High S Cartridges

732-4134
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $271.00
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Bio-Scale Mini Macro-Prep DEAE Cartridges

732-4140
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $215.00
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Bio-Scale Mini Macro-Prep DEAE Cartridge

732-4142
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $76.00
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Bio-Scale Mini Macro-Prep DEAE Cartridges

732-4144
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $281.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev B Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
10006400 Bio-Scale Mini Macro-Prep High Q, High S, and DEAE Cartridges, 1 and 5 ml, Instruction Manual, Rev B Click to download