Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry. The mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.
The cartridges are available with either CHT Type I or Type II, 40 µm media. The Type I support has a protein binding capacity of 25–60 mg of human IgG/ml and higher capacity for acidic proteins. Type II has a protein binding capacity of 15–25 mg of human IgG/ml and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins.
The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:
- Excellent capture at elevated flow rates, enabling processing at all scales
- Large capacity for high-titer upstream feedstocks
- Exceptional selectivity, allowing a two-step chromatographic process
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (request bulletin 5326).
CHT Ceramic Hydroxyapatite Mechanism
Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites are distributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the phosphate groups and/or metal affinity via the calcium atoms.
For more information, request bulletin 5709.
*U.S. patent 7,208,087.