Bio-Scale™ Mini CHT™ Ceramic Hydroxyapatite Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml–40 mm length x 5.6 mm inner diameter
5 ml–40 mm length x 12.6 mm inner diameter
10 ml–80 mm length x 12.6 mm inner diameter**
50 ml–80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

CHT Specifications
  Type I Type II
Functional groups Ca2+, PO4, OH Ca2+, PO4, OH
Observed dynamic binding capacity lysozyme (Lys) ≥25 mg Lys/g CHT ≥12.5 mg Lys/g CHT
Nominal pore diameter 600–800 A 800–1,000 A
Maximum backpressure 100 bar (1,500 psi) 100 bar (1,500 psi)
Nominal mean particle size 20 ± 2, 40 ± 4, and 80 ± 8 µm 20 ± 2, 40 ± 4, and 80 ± 8 µm
Bulk density 0.63 g/ml 0.63 g/ml
CHT Characteristics
  Type I Type II
Observed dynamic binding capacity IgG 25–60 mg IgG/ml CHT* 15–25 mg IgG/ml CHT**
Typical linear flow rate range 50–1,000 cm/hr  
pH Stability 6.5–14 pH  
Base Stability at least 21 months in 1 N NaOH  
Regeneration 500 mM sodium phosphate, pH7
1,000 mM trisodium phosphate, pH 11–12
 
Autoclavability (bulk) 121°C, 20 min in phosphate buffer, pH 7  
Sanitization 1–2 N NaOH  
Recommended column storage 0.1 M NaOH  
Shelf life (dry, unused material) 85 months stored dry, sealed, and at room temperature  

* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5

IgG Protocol
Purification of IgG Using CHT

Purification of IgG using CHT Ceramic Hydroxyapatite.

To read more click here

Acidic Proteins Protocol Using CHT

Acidic Proteins Protocol using CHT
Optimizing the bioprocess requires management of multiple variables ranging from matrix interaction, elution characteristics, scale-up from bench, regulation and drug safety requirements, and process robustness and economics. This protocol has been developed as general starting guidelines for the purification of most proteins and nucleic acids and may help to reduce time spent in methodology development.

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry. The mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The cartridges are available with either CHT Type I or Type II, 40 µm media. The Type I support has a protein binding capacity of 25–60 mg of human IgG/ml and higher capacity for acidic proteins. Type II has a protein binding capacity of 15–25 mg of human IgG/ml and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins.

The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:

  • Excellent capture at elevated flow rates, enabling processing at all scales
  • Large capacity for high-titer upstream feedstocks
  • Exceptional selectivity, allowing a two-step chromatographic process

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (request bulletin 5326).

CHT Ceramic Hydroxyapatite Mechanism

Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites are distributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the phosphate groups and/or metal affinity via the calcium atoms.

For more information, request bulletin 5709.

*U.S. patent 7,208,087.

Bio-Scale Mini CHT Type I Cartridge

732-4322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:   $143.00
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Bio-Scale Mini CHT Type I Cartridges

732-4324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:   $574.00
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Bio-Scale Mini CHT Type II Cartridge

732-4332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:   $143.00
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Bio-Scale Mini CHT Type II Cartridges

732-4334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:   $574.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
10011433 Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges, 5 ml, Instruction Manual, Rev A Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5709 How CHT Ceramic Hydroxyapatite Works, Rev A Click to download [ Add to Cart (Free) ]
5667 CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
2156 CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C Click to download [ Add to Cart (Free) ]
5739 Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A Click to download [ Add to Cart (Free) ]
2731 Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A Click to download
2780 Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A Click to download [ Add to Cart (Free) ]
2524 Purification of Horse IgGT Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C Click to download
2774 Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A Click to download
1986 Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B Click to download [ Add to Cart (Free) ]
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download [ Add to Cart (Free) ]
2940 Removal of Aggregate From an IgG4 Product Using CHT Ceramic Hydroxyapatite, Rev A Click to download
2849 Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A Click to download
2535 Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B Click to download
2575 Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite Click to download
2946 Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up, Rev C Click to download [ Add to Cart (Free) ]
6530 Protein Purification Workflow Development Using Bio-Rad’s NGC Chromatography System, Rev B Click to download