Bio-Scale™ Mini CHT™ 40 µm Cartridges

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Overview

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Bio-Scale Mini CHT cartridges are 5 ml Bio-Scale Mini cartridges filled with either CHT Type I or Type II having a particle size of 40 µm:

  • Type I — sintered at 400°C; has a high protein binding capacity (>25 mg of lysozyme/g) and higher capacity for acidic proteins
  • Type II — sintered at 700°C; has a lower protein binding capacity (>12.5 mg of lysozyme/g) and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

* U.S.patent 7,208,087.

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  
Bio-Scale Mini CHT Type I Cartridge

732-4322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:   $143.00
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Bio-Scale Mini CHT Type I Cartridges

732-4324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:   $574.00
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Bio-Scale Mini CHT Type II Cartridge

732-4332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:   $143.00
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Bio-Scale Mini CHT Type II Cartridges

732-4334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:   $574.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
1929 Bio-Scale Ceramic Hydroxyapatite Type I Column Product Information Sheet, Rev A Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
10011433 Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges, 5 ml, Instruction Manual, Rev A Click to download [ Add to Cart (Free) ]
6484 Bio-Rad’s High-Performance Columns for Antibody and Tagged Protein Purification, Rev A Click to download