Bio-Scale Mini CFT ceramic fluoroapatite cartridges are 5 ml cartridges filled with CFT ceramic fluoroapatite support. CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent. CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5.6. The cartridges can be used for method scouting or simple purifications.
CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.
Features of CFT include:
- High binding capacity
- Sintering at 700°C (Type II) for heavy-duty, durable supports
- Rigid particles for fast cleaning and equilibration
- Inorganic calcium phosphate for distinct selectivities
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connection to any system easy (see bulletin 5326).
*U.S. patent 7,208,087.