Bio-Scale Mini Affi-Prep protein A cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with Affi-Prep protein A support. Affinity chromatography on this support yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. Advantages include:
- High purity of IgG
- High affinity for mammalian IgG
- High capacities for mouse IgG1 as well as other subclasses with optimized MAPS buffer solutions
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.
* U.S. patent 7,208,087.