Bio-Scale™ Mini Affi-Gel® Blue Cartridges

Print

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A Affi-Prep® Protein A Affinity Recombinant protein A 30 mg/ml human IgG
732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml Bio-Scale Mini cartridges filled with 100–200 mesh Affi-Gel Blue support for use in affinity chromatography. This form of Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached cibacron blue F3GA dye that is ideally suited for albumin removal. Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of eluent buffers.

Bio-Scale Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts.

Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

* U.S. patent 7,208,087.

Size 5 ml bed volume
Dimensions (L x ID)*  40 x 12.6 mm
Maximum pressure tolerance 10 psi (680 mbar, 0.26mPa)**
Operational flow rates 0.5–2.0 ml/min (25–100 cm/hr)
Fittings Female luer fitting inlet; male luer fitting outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping conditional 0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Storage recommendations 0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Autoclavability Not autoclavable
* L, length; ID, inner diameter.  
** Although the pressure limitation of the cartridge is 45 psi, the agarose gel tends to compress above 10 psi (generally 3.0 ml/min).  
Bio-Scale Mini Affi-Gel Blue Cartridge

732-4642
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $128.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Scale Mini Affi-Gel Blue Cartridges

732-4644
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $451.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
1 ml Empty Bio-Scale™ Mini Cartridges

732-4660
Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

List Price:   $55.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
5 ml Empty Bio-Scale™ Mini Cartridges

732-4661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

List Price:   $112.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev B Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
10006402 Bio-Scale Mini Affi-GelĀ® Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev C Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]