Bio-Rad® InPlace™ Columns

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Overview

Bio-Rad® InPlace™ Column Video


Bio-Rad InPlace process-scale chromatography columns are designed for industrial applications and allow contained filling, packing, unpacking, and cleaning in place for safe and hygienic column operation. To learn more about Bio-Rad InPlace columns, click on the video.

Innovative Features of the Bio-Rad® InPlace™ Column

Slurry transfer methods. A, pump-mediated transfer using diaphragm pumps of the Bio-Rad media transfer device; B, vacuum-mediated transfer uses the motorized piston as a large syringe to move slurry from the tank to the column by suction.

For more information about the Bio-Rad InPlace column, request or download bulletin 6091.

Sanitization of the Bio-Rad® InPlace™ Column

Process chromatography columns must provide a very high level of cleanability to meet the requirements of various regulatory agencies. The efficiency of the column cleaning process must satisfy regulations, norms, and standards, such as cGMP, Norm ISO 13408, FDA 21-211.67, or USP 32 NF27.

Bio-Rad InPlace column and control console.

For more information about sanitization of the Bio-Rad InPlace column, request or download bulletin 6061.

Specifications*
      Maximum Pressure at 30˚C, bar
Nominal Internal Diameter, mm** Actual Internal Diameter, mm Motorization Available Calibrated Borosilicate Glass Stainless Steel 316L*** Acrylic***
180 180 ± 2 Yes 5.2 6.0 6.0
200 200 ± 2 Yes 4.7 6.0 6.0
250 250 ± 2 Yes 3.8 6.0 6.0
300 296 ± 2 Yes 3.6 6.0 6.0
350 350 ± 2 Yes 6.0 6.0
400 400 ± 2 Yes 6.0 6.0
450 446 ± 2 Yes 2.4 6.0 6.0
500 500 ± 2 Yes 6.0 6.0
600 596.5 ± 2 Yes 6.0 6.0
700 700 ± 2 Yes 3.0 3.0
800 800 ± 2 Yes 3.0 3.0
1,000 1,000 ± 3 Yes 3.0 3.0
1,200 1,200 ± 3 Yes 3.0 3.0
1,300 1,300 ± 3 Yes 3.0 3.0
1,400 1,400 ± 3 Yes 3.0 3.0

* Standard tube heights available: 600 and 900 mm.
** Columns with internal diameters >1,400 mm are available on request.
*** Higher pressure rating can be quoted on request.
– Not available.

Bio-Rad® InPlace™ Column Schematic
InPlace Schematic

Schematic drawing of the Bio-Rad InPlace column.

Flow Distribution
Flow Distribution

Bio-Rad InPlace process-scale chromatography columns are designed for industrial applications and allow contained filling, packing, unpacking, and cleaning in place. The columns have a sanitary design, variable height adaptor, piston motor, inflatable seal, and special low-shear valves for safe slurry transfer. The piston motors enable high-quality, reproducible packing by axial compression. Axial compression may be combined with flow packing to meet process requirements. Specially designed inlet valves minimize mechanical stress on chromatography media during loading and unloading.

Bio-Rad InPlace Columns

Bio-Rad InPlace columns are designed so that the packing, unpacking, and cleaning-in-place features do not interfere with the bed support, distribution system, and column tube, allowing optimum performance during any operation. These columns are directly scalable and retain the same general design from pilot to manufacturing scale.

Key Features

  • Closed system design
  • Ideal for dense media
  • Adjustable height adaptor for axial compression
  • Designed for multiple packing methods
  • No resin loss during packing
  • Low mechanical shear during media transfer
  • Chromatography tubes available in glass, acrylic, and stainless steel
  • Hoist-free operation with the Bio-Rad® MainFrame™ lifting accessory
  • Complete documentation for regulatory submissions
  • 18 cm–2 m size range

Bio-Rad InPlace Control Console

The Bio-Rad InPlace control console provides automation features for stand-alone InPlace process chromatography columns ranging in diameters from 18 cm to 2 m using conventional skids and media transfer. Existing InPlace columns can be upgraded to the automated InPlace control console.

Key Features

  • Simplifies column operations using preprogrammed methods for column filling, bed consolidation, unpacking, and cleaning
  • Ensures precise packing, speed, and bed height adjustment at each step
  • Manages all programming using an intuitive touch-screen interface onboard maintenance guide

Each item is delivered with CE certification and is manufactured in our facility, which is certified according to ISO 9001:2000 standards. In Bio-Rad's manufacturing procedures, all steps and components are fully documented. The qualification documentation package ensures production in compliance with U.S. and European regulations.

Number Description Options
5739 Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A Click to download [ Add to Cart (Free) ]
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up, Rev C Click to download [ Add to Cart (Free) ]
6042 Bio-Rad InPlace Control Console Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5663 Bio-Rad InPlace Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5665 Bio-Rad EasyPack Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5997 Bio-Rad Process Chromatography Skid 00 Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
6043 Bio-Rad Chromatography Station 00 Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6091 Innovative Features of Bio-Rad InPlace Chromatography Columns Simplify Packing Procedures for Any Media Type, Rev A Click to download [ Add to Cart (Free) ]
5661 Bio-Rad Media Transfer Device, Rev B Click to download [ Add to Cart (Free) ]
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
5659 Bio-Rad Mainframe Lifting Accessory Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5488 State-of-the-Art Solutions for Process-Scale Chromatography, Rev C Click to download [ Add to Cart (Free) ]
5614 Bio-Rad Bubble Trap Product Information Sheet, Rev B Click to download
5618 Pressure Gauge Product Information Sheet, Rev A Click to download
5620 Bio-Rad Media Slurry Tank Product Information Sheet, Rev A Click to download
5619 Pressure Relief Valve Product Information Sheet, Rev A Click to download
5615 Isolation Two-Way Valves Product Information Sheet, Rev A Click to download
5616 Liquid Pump for Inflatable Seals Product Information Sheet, Rev B Click to download
5617 Bio-Rad Packing Motor Product Information Sheet, Rev A Click to download
6061 Sanitization of a Packed Bed in the Bio-Rad InPlace Process Chromatography Column, Rev A Click to download [ Add to Cart (Free) ]