Bio-Plex System Validation and Calibration Tools

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Overview

Specifications
Parameter At High PMT Target Value At Low PMT Target Value
Optics Validation
DD median 4,774–6,593 4,774–6,593
CL1 median 3,383–4,135 3,383–4,135
CL1 CV% 2.0–7.0% 2.0–7.0%
CL2 median 3,520–4,302 3,520–4,302
CL2 CV% 3.0–8.0% 3.0–8.0%
RP1 median 15,205–18,583 3,509–4,290
RP1 CV% 4.0–10.0% 4.0–10.0%
Fluidics Validation
% Carryover ≤4.0% ≤4.0%
Reporter Validation
Dynamic range 4.19–4.39 4.33–4.45
Linearity >0.995 >0.995
Slope 127.3–207.0 33.9–39.4
Accuracy >90.0% >90.0%
Instrument threshold <20 MFI <6 MFI
Classify Validation
Bead A (region 1) >80.0% >80.0%
Bead B (region 4) >80.0% >80.0%
Bead C (region 40) >80.0% >80.0%
Bead D (region 54) >80.0% >80.0%
Bead E (region 100) >80.0% >80.0%
Doublet discrimination efficiency
 ≥75.0%  ≥75.0%



System performance validation and calibration tools for the Bio-Plex 200 system ensure reliability and consistency of assay results. Both validation and calibration protocols are automated by Bio-Plex Manager software, and records are stored in a secure Bio-Plex database file. Procedures are performed using a maintenance, calibration, and validation (MCV) plate that holds the required bead sets and solutions.

Bio-Plex validation and calibration kits, Bio-Plex Manager software, and the Bio-Plex MCV plate IV enable you to:

  • Perform routine instrument maintenance, calibration, and validation procedures
  • Assess and document the performance of all system components
  • Discriminate between instrument and assay problems

Bio-Plex Validation Kit 4.0 and Bio-Plex MCV Plate IV
The Bio-Plex validation kit 4.0 includes bead sets for validation routines (optics, reporter, classify, and fluidics validation) using Bio-Plex Manager software and the Bio-Plex MCV plate IV.

Key features include:

  • Automated validation with both high and low PMT settings
  • Broadened classification regions from region 1 to 100
  • Bio-Plex flashlight for visual inspection and adjustment of sample-needle height
  • Two needle-height adjustment options to accommodate various plate types
  • Bio-Plex reservoir to enable unattended operation of maintenance functions

Bio-Plex Calibration Kit
The Bio-Plex calibration kit consists of a set of gold standard calibration beads with stable fluorescent signals. Calibration standardizes signal output from day to day and from instrument to instrument. The Bio-Plex system allows calibration at two different settings. The calibration for broad range standards provides the widest available dynamic range, while the calibration for low range standards with Bio-Plex Manager software StatLIA curve fitting provides the greatest sensitivity.

Bio-Plex® Validation Kit 4.0

171-203001
Performance testing tool for Bio-Plex 200 system; works with Bio-Plex Manager 4.0, 4.1.1, 5.0, or 6.0 software and (uses MCV plate IV); ~50 validation routines

List Price:   $1,660.00
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Bio-Plex Calibration Kit

171-203060
Performance testing tool for Bio-Plex 200 system, includes Cal1 and Cal2 calibration beads; ~50 daily calibration routines

List Price:   $543.00
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Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
3076 Bio-Plex Care and Maintenance Quick Guide, Rev B Click to download
2993 Bio-Plex Glossary, Rev A Click to download
4100205 Bio-Plex Maintenance Log, Rev A Click to download [ Add to Cart (Free) ]
3000 Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A Click to download
4110016 Instruction Manual, Bio-Plex Calibration Kit Product Insert, Rev B Click to download
4110007 Instruction Manual, Bio-Plex Validation Kit 3.0, Rev C Click to download
4110017 Instruction Manual, Bio-Plex Validation Kit 3.0 Specification Sheet, Rev B Click to download
4110185 Instruction Manual, Bio-Plex Validation Kit 4.0, Rev B Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
2879 Bio-Plex System Tools Brochure, Rev C Click to download