Bio-Plex Pro™ Mouse Cytokine, Chemokine, and Growth Factor Assays

Print
Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Bio-Plex Pro™ Mouse Cytokine Assays

Mouse
Group I
8-Plex
Panel
(M60-
000007A
)
23-Plex
Panel
(M60-
009RDPD
)
Th1/Th2
Panel
(M60-
00003J7
)
Th1
Panel
(L60-
00004C6
)
Th2
Panel
(L60-
000UKVT
)
Th17
6-Plex
Panel
(M60-
00007NY
)
Singleplex Bead Regions*
IL-1α        
171-G5001M 53
IL-1β     171-G5002M 19
IL-2   171-G5003M 36
IL-3           171-G5004M 18
IL-4     171-G5005M 39
IL-5     171-G5006M 52
IL-6     171-G5007M 38
IL-9         171-G5008M 33
IL-10 171-G5009M 56
IL-12 p40           171-G5010M 76
IL-12 p70       171-G5011M 78
IL-13         171-G5012M 37
IL-17A         171-G5013M 72
Eotaxin           171-G5014M 74
G-CSF           171-G5015M 54
GM-CSF       171-G5016M 73
IFN-γ  •   171-G5017M 34
KC           171-G5018M 57
MCP-1 (MCAF)           171-G5019M 51
MIP-1α           171-G5020M 77
MIP-1β           171-G5021M 75
RANTES           171-G5022M 55
TNF-α   171-G5023M 21

 

Mouse Group II 9-Plex Panel
(MD0-00000EL)
Singleplex Bead Regions*
IL-15 171-G6001M 42
IL-18**   20
Basic FGF 171-G6002M 25
LIF 171-G6003M 45
M-CSF 171-G6004M 26
MIG 171-G6005M 44
MIP-2 171-G6006M 27
PDGF-BB 171-G6007M 35
VEGF 171-G6008M 47

 

Mouse Group III 8-Plex Panel
(171-FA001M)
Th17 Singleplex Assays Bead Regions*
CD40L 171-GA001M 30
IL-17F 171-GA002M 28
IL-21 171-GA003M 14
IL-22 171-GA004M 15
IL-23p19 171-GA005M 61
IL-25 (IL-17E)   171-GA006M 67
IL-27p28   171-GA007M 43
IL-31 171-GA008M 29
IL-33 171-GA009M 13
ICAM-1   171-GA010M 22
MIP-3α 171-GA011M 12

TGF-β Group 3-Plex Panel
(171-W4001M)
Singleplex Bead Regions
TGF-β1 171-V4001M 13
TGF-β2 171-V4002M 72
TGF-β3 171-V4003M 66


* You can design an x-Plex assay or Express assay with any combination of analytes within a group, except for ICAM-1.

** IL-18 is not available as a singleplex assay but only as part of an assay panel.

Note: Red bead regions indicate regions that differ between magnetic and nonmagnetic assay targets.

Bio-Plex Pro™ Mouse Cytokine Assay Specifications

Representative working assay range*, sensitivity, and precision**. Values were experimentally determined from five assay runs.

  Assay Working Ranges pg/ml Assay Sensitivity pg/ml Assay Precision
Targets LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV
Mouse Group I: 23-plex panel
IL-1α 1.84 21,093 0.2 3 29
IL-1β 10.36 60,631 9.4 4 7
IL-2 3.72 51,857 0.6 3 19
IL-3 1.55 21,632 0.2 2 15
IL-4 6.98 9,372 2.1 3 20
IL-5 3.57 13,315 0.3 4 14
IL-6 0.74 12,053 0.2 3 16
IL-9 6.89 28,208 12.5 4 9
IL-10 2.95 12,066 1.0 4 5
IL-12 (p40) 1.53 25,024 0.4 2 7
IL-12 (p70) 1.62 26,507 2.3 3 7
IL-13 47.2 57,011 38.7 4 5
IL-17A 2.65 43,337 0.8 3 10
Eotaxin 257.9 4,636 147.4 4 5
G-CSF 5.1 84,244 0.6 3 12
GM-CSF 21.2 3,401 5.6 3 24
IFN-γ 1.84 30,164 1.2 4 6
KC 3.2 18,202 0.3 3 30
MCP-1 22.4 41,873 3.7 5 7
MIP-1α 256.2 15,565 36.3 3 11
MIP-1β 3.33 24,798 0.8 3 8
RANTES 2.78 8,759 0.6 4 4
TNF-α 5.8 59,626 1.4 3 6
Mouse Group II: 9-plex panel
IL-15 5.7 37,501 6.6 6 3
IL-18 81.4 29,761 31.8 8 18
Basic FGF 4.8 35,500 2.2 8 8
LIF 3.5 57,366 0.6 4 12
M-CSF 1.5 24,221 0.4 4 14
MIG 183 46,393 3.4 5 21
MIP-2 32 8,574.2 0.4 5 13
PDGF-BB 10.4 36,939 4.3 5 10
VEGF 27.2 32,771 1.6 4 15

* Assay range is LLOQ and ULOQ calculated from five independent assays.

** Data were generated using vacuum manifold. %CV is expected to be lower with magnetic bead washer.

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

Bio-Plex Pro™ Mouse Th17 Assay Specifications
  Assay Working Ranges pg/ml Assay Sensitivity pg/ml Assay Precision
  Target LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV
8-Plex IL-17F 1 6,362 0.1 5 5
IL-21 6 8,207 2.8 3 15
IL-22 0.5 8,433 0.1 4 5
IL-23p19 3.4 55,764 0.7 5 6
IL-31 2.5 41,313 5.2 6 6
IL-33 0.5 7,343 0.2 5 12
CD40L 3.5 11,245 0.4 4 14
MIP-3α 0.7 1,660 0.3 6 13
 
6-Plex IL-1α 10.36 60,631 9.4 4 7
IL-6 0.74 12,053 0.2 3 16
IL-10 2.95 12,066 1 4 5
IL-17A 2.65 43,337 0.8 3 10
IFN-γ 1.84 30,164 1.2 4 6
TNF-α 5.8 59,626 1.4 3 6
 
2-Plex IL-25 168 98,266 9 9 12
IL-27p28 63.4 117,521 29.2 9 6
 
Singleplex ICAM-1 2.9 23,723 0.78 4 8

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (limit of detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal

TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV

   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
4.9
TGF-β2
14.7 
30,080 1.9
6.3 
9.1
TGF-β3
2.8 
15,031 0.5
6.9 
8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex Assay Builder

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder



Bio-Plex® Technology and Applications

Bio-Plex Pro™ mouse cytokine, chemokine, and growth factor magnetic bead–based assays detect and measure levels of 46 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kit
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with Bio-Plex Pro mouse diabetes assays for an expanded biomarker profile

Flexible Ordering Options

For multiplexing capabilities among cytokines, refer to bulletin 5927. For multiplexing capabilities with diabetes assays, refer to bulletin 5975.

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Luminex MAGPIX system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Bio-Plex Pro™ Mouse Cytokine Th17 Panel A 6-Plex Group l

M60-00007NY
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 6 mouse cytokines all in one kit

List Price:   $1,230.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro™ Mouse Cytokine Th17 Panel B 8-Plex Group III

171-FA001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

List Price:   $1,757.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro™ Mouse Cytokine Th1 Panel

L60-00004C6
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 7 mouse cytokines all in one kit

List Price:   $2,072.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro™ Mouse Cytokine Th2 Panel

L60-000UKVT
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 7 mouse cytokines all in one kit

List Price:   $2,072.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro Mouse Cytokine 8-plex Assay #M60-000007A
Bio-Plex Pro™ Mouse Cytokine 8-plex Assay

M60-000007A
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

List Price:   $1,527.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro Mouse Cytokine Th1/Th2 Assay #M60-00003J7
Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay

M60-00003J7
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

List Price:   $1,537.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro Mouse Cytokine 23-plex Assay #M60-009RDPD
Bio-Plex Pro™ Mouse Cytokine 23-plex Assay

M60-009RDPD
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 23 mouse cytokines all in one kit

List Price:   $3,285.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro™ Mouse Cytokine 9-Plex Assay

MD0-00000EL
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 9 mouse cytokines all in one kit

List Price:   $1,783.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Plex Pro™ TGF-β 3-plex Assay

171-W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, sealing tape, for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

List Price:   $866.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6110 Bio-Plex Pro Mouse Th17 Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D Click to download
10024985 Instruction Manual, Bio-Plex Pro Assays Quick Guide 4, Rev A Click to download
3100 Bio-Plex Cytokine Assay Comparison, Rev E Click to download
5297 Bio-Plex Cytokine Assay References, Rev C Click to download
2993 Bio-Plex Glossary, Rev A Click to download
3074 Differential Immune Activation in Astrocytes of IL-6 Genotypes, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
2900 Selection of Standards for Bio-Plex Cytokine Assays, Rev A Click to download
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5975 Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays, Rev A Click to download
5927 Multiplexing Across Cytokine Panels, Rev A Click to download
5800 Development and Validation of Two Magnetic Bead-Based Mouse Cytokine Multiplex Assay Panels, Rev A Click to download
5827 Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Assays Product Sheet, Rev C Click to download [ Add to Cart (Free) ]