Bio-Plex Pro™ Magnetic Cell Signaling Assays

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Bio-Plex Pro Magnetic Cell Signaling Assays

Overview

Available Magnetic Assays

Housekeeping Proteins   Total Protein Target Assays Bead Region
β-actin 171-V60020M 47
GAPDH 171-V60019M 21

Phosphoprotein Assays   Total Protein Target Assays Bead Region
Akt (Ser473) 171-V50001M Akt 171-V60001M 75
Akt (Thr308) 171-V50002M
ATF-2 (Thr71) 171-V50024M     20
BAD (Ser136) 171-V50025M     26
Btk (Tyr223) 171-V50026M Btk 171-V60012M 39
c-Abl (Tyr245) 171-V50027M     45
c-Jun (Ser63) 171-V50003M c-Jun 171-V60002M 56
CREB (Ser133) 171-V50028M CREB 171-V60013M 19
EGFR (Tyr1068) 171-V50004M     44
EGFR (Tyr1173) 171-V50005M     44
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) 171-V50006M Erk1/2 171-V60003M 38
GSK-3α/β (Ser21/Ser9) 171-V50007M GSK-3β 171-V60004M 18
HER-2 (Tyr1248) 171-V50008M HER-2 171-V60005M 30
HSP27 (Ser78) 171-V50029M     51
IGF-1R (Tyr1131) 171-V50009M IGF-1R 171-V60014M 43
IR-β (Tyr1146) 171-V50031M     43
IRS-1 (Ser636/Ser639) 171-V50030M     76
IκB-α (Ser32/Ser36) 171-V50010M IκB-α 171-V60006M 67
JNK (Thr183/Tyr185) 171-V50011M JNK 171-V60007M 34
Lyn (Tyr507) 171-V50032M     33
MEK1 (Ser217/Ser221) 171-V50012M MEK1 171-V60008M 27
mTOR (Ser2448) 171-V50033M mTOR 171-V60015M 46
NF-κB p65 (Ser536) 171-V50013M     37
p38 MAPK (Thr180/Tyr182) 171-V50014M p38 MAPK 171-V60009M 36
p53 (Ser15) 171-V50034M     53
p70 S6 kinase (Thr421/Ser424) 171-V50015M p70 S6 kinase 171-V60010M 55
p70 S6 kinase (Thr389) 171-V50016M
p90 RSK (Ser380) 171-V50035M     35
PDGFR-α (Tyr754) 171-V50017M     28
PDGFR-β (Tyr751) 171-V50018M     57
PI3K p85 (Tyr458) 171-V50036M     54
PTEN (Ser380) 171-V50037M PTEN 171-V60016M 22
S6 ribosomal protein (Ser235/Ser236) 171-V50038M     74
Smad2 (Ser465/Ser467) 171-V50019M Smad2 171-V60011M 14
Src (Tyr416) 171-V50039M Src 171-V60017M 42
Stat1 (Tyr701) 171-V50020M     61
Stat3 (Ser727) 171-V50021M     52
Stat3 (Tyr705) 171-V50022M     52
Syk (Tyr352)       171-V50040M     65
VEGFR-2 (Tyr1175) 171-V50023M     29
ZAP-70 (Tyr319) 171-V50041M ZAP-70 171-V60018M 64

 

 

CST antibodies developed and validated for Bio-Plex phosphoprotein and total target assays.


Multiplexability Matrix
Assays Cross-Reactivity >10%
β-actin Akt (Ser473), Akt (Thr308), Stat3 (Tyr705)
Akt (total)
Smad2 (total)
Akt (Ser473) β-actin, Akt (Thr308)
Akt (Thr308) β-actin, Akt (Ser473), ATF-2 (Thr71), Stat3 (Tyr705)
ATF-2 (Thr71) Akt (Thr308), c-Jun (Ser63)
Btk (Tyr223) HER-2 (Tyr1248), Lyn (Tyr507), PI3K p85 (Tyr458), Src (Tyr416)
c-AbI (Tyr245) EGFR (Tyr1173), IR-β (Tyr1146), PI3K p85 (Tyr458)
c-Jun (Ser63) ATF-2 (Thr71), P70 S6 kinase (Thr421/Ser424)
EGFR (Tyr1068)
EGFR (Tyr1173), HER-2 (Tyr1248), IGF-1R (Tyr1131), Stat1 (Tyr701), Stat3 (Tyr705)
EGFR (Tyr1173) c-Abl (Tyr245), EGFR (Tyr1068), HER-2 (Tyr1248), IGF-1R (Tyr1131), Stat1 (Tyr701)
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) HER-2 (Tyr1248)
HER-2 (Tyr1248) Btk (Tyr223), EGFR (Tyr1068), EGFR (Tyr1173), Erk1/2 (Thr202/Tyr204,Thr185/Tyr187), IGF-1R (Tyr1131), Lyn (Tyr507), PDGFR-α (Tyr754), PDGFR-β (Tyr751), PI3K p85 (Tyr458), Stat1 (Tyr701)
IGF-1R (Tyr1131) EGFR (Tyr1068), EGFR (Tyr1173), HER-2 (Tyr1248), IR-β (Tyr1146), PDGFR-α (Tyr754),
PDGFR-β (Tyr751)
IR-β (Tyr1146) c-Abl (Tyr245), IGF-1R (Tyr1131), Lyn (Tyr507), PI3K p85 (Tyr458), Src (Tyr416), ZAP-70 (Tyr319)
IRS-1 (Ser636/Ser639) PI3K p85 (Tyr458)
IκB-α (Ser32/Ser36) NF-κB p65 (Ser536)
Lyn (Tyr507) Btk (Tyr223), HER-2 (Tyr1248), IR-β (Tyr1146), PI3K p85 (Tyr458)
MEK1 (Ser217/Ser221) P70 S6 kinase (Thr389), P70 S6 kinase (Thr421/Ser424)
NF-κB p65 (Ser536) IκB-α (Ser32/Ser36), Stat3 (Tyr705)
p38 MAPK (total) c-Jun (total)
p70 S6 kinase (Thr389) MEK1 (Ser217/Ser221), p70 S6 kinase (Thr421/Ser424)
p70 S6 kinase (Thr421/Ser424) c-Jun (Ser63), MEK1 (Ser217/Ser221), p70 S6 kinase (Thr389)
PDGFR-α (Tyr754) HER-2 (Tyr1248), IGF-1R (Tyr1131), PDGFR-β (Tyr751)
PDGFR-β (Tyr751)
HER-2 (Tyr1248), IGF-1R (Tyr1131), PDGFR-α (Tyr754)
Pl3K p85 (Tyr458)
Btk (Tyr223), c-Abl (Tyr245), HER-2 (Tyr1248), IR-β (Tyr1146), IRS-1 (Ser636/Ser639), Lyn (Tyr507),
Syk (Tyr352), ZAP-70 (Tyr319)
Src (Tyr416) Btk (Tyr223), IR-β (Tyr1146)
Stat1 (Tyr701) EGFR (Tyr1068), EGFR (Tyr1173), HER-2 (Tyr1248), Stat3 (Ser727), Stat3 (Tyr705)
Stat3 (Ser727) Stat1 (Tyr701), Stat3 (Tyr705)
Stat3 (Tyr705) β-actin, Akt (Thr308), EGFR (Tyr1068), NF-κB p65 (Ser536), Stat1 (Tyr701), Stat3 (Ser727)
Syk (Tyr352) PI3K p85 (Tyr458)
ZAP-70 (Tyr319) IR-β (Tyr1146), PI3K p85 (Tyr458)

 

Bio-Rad Cell Lysate Controls* Selection Guide

Phosphoprotein Lysate Control Catalog #
Akt (Ser473)
Akt (Thr308)
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)
GSK-3α/β (Ser21/Ser9)
MEK1 (Ser217/Ser221)
EGF-treated HEK-293 171-YZ0001
ATF-2 (Tyr71)
c-Jun (Ser63)
CREB (Ser133)
JNK (Thr183/Tyr185)
p38 MAPK (Thr180/Tyr182)
p53 (Ser15)
UV-treated HEK-293 171-YZ0009
BAD (Ser136)
IRS-1 (Ser636/Ser639)
mTOR (Ser2448)
PDGFR-α (Tyr754)
PDGFR-β (Tyr751)
PTEN (Ser380)
PDGF-treated NIH3T3 171-YZ0007
Btk (Tyr223)
Lyn (Tyr507)
Pl3K p85 (Tyr458)
Syk (Tyr352)
H2O2-treated Ramos 171-YZ0011
c-AbI (Tyr245) Untreated K-562 171-YZT003
EGFR (Tyr1068)
EGFR (Tyr1173)
EGF-treated HeLa 171-YZ0002
HER-2 (Tyr1248)
HSP27 (Ser78)
p90 RSK (Ser380)
S6 ribosomal protein (Ser235/Ser236)
EGF-treated SK-Br3 171-YZ0003
IGF-1R (Tyr1131)
IR-β (Tyr1146)
IGF-1-treated HEK-293 171-YZ0005
IκB-α (Ser32/Ser36)
NF-κB p65 (Ser536)
Smad2 (Ser465/Ser467)
TNF-α-treated HeLa 171-YZ0008
p70 S6 kinase (Thr421/Ser424)
p70 S6 kinase (Thr389)
NGFβ-treated PC12 171-YZ0006
Src (Tyr416) Src-transfected NIH3T3 171-YZ0013
Stat1 (Tyr701)
Stat3 (Ser727)
Stat3 (Tyr705)
IFN-α-treated HeLa 171-YZ0004
VEGFR-2 (Tyr1175) VEGF-treated HUVEC 171-YZ0010
ZAP-70 (Tyr319) H2O2-treated Jurkat 171-YZ0012
Negative control for
all phosphoprotein assays
Phosphatase-treated HeLa 171-YZB001
Total Target Lysate Control Catalog #
Total Akt
Total Erk1/2
Total GSK-3β
Total IκB-α
Total JNK
Total MEK1
Total mTOR
Total p38 MAPK
Total p70 S6 kinase
Total PTEN
Total Smad2
Total IGF-1R
Untreated HeLa 171-YZT002
Total Btk H2O2-treated Ramos 171-YZ0011
Total ZAP-70 H2O2-treated Jurkat 171-YZ0012
Total c-Jun
Total CREB
Untreated HEK-293 171-YZT001
Total HER-2 EGF-treated SK-Br3 171-YZ0003
Total Src Src-transfected NIH3T3 171-YZ0013
Negative control for
all total target assays
Detection antibody diluent**  
Housekeeping Protein Lysate Control Catalog #
β-actin
Human GAPDH
Untreated HeLa 171-YZT002
Negative control for all housekeeping protein assays Detection antibody diluent**  

* All controls are shipped as one vial of lyophilized cell lysate.

** Detection antibody diluent is included with the Bio-Plex Pro cell signaling reagent kit (171-304006M).

Bio-Plex Assay Builder

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Bio-Plex® Technology and Applications

Bio-Plex Pro magnetic cell signaling assays are multiplexed immunoassays for the detection of intracellular phosphoproteins and total target proteins in cell and tissue lysates. These assays utilize tailored antibodies bound on 6.5 µm magnetic beads. Bio-Plex Pro total target assays measure the total abundance of each target protein, regardless of its phosphorylation state. Bio-Plex Pro phosphoprotein assays measure the relative amounts of phosphorylated proteins only. When both phosphoprotein and total protein assays are performed, they must be run in different wells due to shared bead regions. Using the multiplex format, the profiles of multiple proteins involved in cell signaling pathways can be detected using only 50 µl of lysate.

Features and Benefits of Magnetic Cell Signaling Assays

  • Compatible singleplex sets can be combined to create your own multiplex assay
  • Assay protocol optimized for high sensitivity and broad dynamic range
  • Highly specific antibodies developed and validated by Cell Signaling Technology, Inc.
  • Higher specificity sandwich format that minimizes cross-reactivity with other cellular proteins
  • Low sample volume requirement
  • Minimal hands-on time
  • Assay quick guide to get you started right away

Applications and Uses of Magnetic Cell Signaling Assays

The capacity for multiplexed biomarker analysis makes the Bio-Plex® multiplex immunoassay system the ideal platform for signal transduction assays. Bio-Plex Pro magnetic cell signaling assays can be used to determine intracellular levels of phosphorylated proteins in the investigation of diverse biological processes including apoptosis, translation control, gene expression, cell cycle regulation, cytoskeletal restructuring, and neurological function.

Required Reagents

No additional reagents are needed when purchasing a preconfigured Bio-Plex Pro cell signaling panel. When ordering singleplex sets, a Bio-Plex Pro cell signaling reagent kit (#171-304006M) is needed to perform an assay.

Recommended Reagents

For best results it is recommended that the appropriate cell lysate controls be included in each experiment. Refer to the Bio-Rad cell lysate controls selection guide to identify the appropriate controls for your Bio-Plex Pro cell signaling phosphoprotein or total target assay of interest.

Other Assay Formats Available

Custom assays available as Bio-Plex® x-Plex™ assays

Instrument Compatibility

More Information

Other Bio-Plex magnetic bead–based immunoassays are available for diverse fields of research:

Learn more about the applications of multiplexing immunoassays.

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Reactive species Human, mouse, rat
Matrices Cell culture lysate, tissue lysate
Limit of detection ≤10 µg/total protein
Precision Intra-assay CV <15%
Inter-assay CV <25%

Values vary by assay.

Bio-Plex Pro™ Cell Signaling MAPK Panel, 9-plex

LQ0-0000S6KL81S
1 x 96-well plate, includes coupled magnetic beads and detection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals for the detection of 9 MAPK pathway markers

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Bio-Plex Pro™ Cell Signaling Akt Panel, 8-plex

LQ0-0006JK0K0RR
1 x 96-well plate, includes coupled magnetic beads and detection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals for the detection of 8 Akt pathway markers

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Bio-Plex Pro Phospho Akt (Ser473) Set (#171-V50001M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Akt (Ser473) Set

171-V50001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Akt (Ser473), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Akt (Thr308) Set (#171-V50002M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Akt (Thr308) Set

171-V50002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Akt (Thr308), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-ATF-2 (Thr71) Set

171-V50024M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated ATF-2 (Thr71), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-BAD (Ser136) Set

171-V50025M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated BAD (Ser136), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-Btk (Tyr223) Set

171-V50026M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Btk (Tyr223), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-c-Abl (Tyr245) Set

171-V50027M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated c-Abl (Tyr245), requires reagent kit and lysates

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Bio-Plex Pro Phospho-c-Jun (Ser63) Set (#171-V50003M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-c-Jun (Ser63) Set

171-V50003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated c-Jun (Ser63), requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Phospho-CREB (Ser133) Set

171-V50028M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated CREB (Ser133), requires reagent kit and lysates

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Bio-Plex Pro Phospho-EGFR (Tyr1068) Set (#171-V50004M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-EGFR (Tyr1068) Set

171-V50004M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated EGFR (Tyr1068), requires reagent kit and lysates

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Bio-Plex Pro Phospho-EGFR (Tyr1173) Set (#171-V50005M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-EGFR (Tyr1173) Set

171-V50005M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated EGFR (Tyr1173), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) Set (#171-V50006M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) Set

171-V50006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Erk1/2 (Thr202/Tyr204, Thr185/Tyr187), requires reagent kit and lysates

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Bio-Plex Pro Phospho-GSK-3α/ß (Ser21/Ser9) Set (#171-V50007M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-GSK-3α/β (Ser21/Ser9) Set

171-V50007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated GSK-3α/β (Ser21/Ser9), requires reagent kit and lysates

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Bio-Plex Pro Phospho-HER-2 (Tyr1248) Set (#171-V50008M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-HER-2 (Tyr1248) Set

171-V50008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated HER-2 (Tyr1248), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-HSP27 (Ser78) Set

171-V50029M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated HSP27 (Ser78), requires reagent kit and lysates

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Bio-Plex Pro Phospho-IGF-IR (Tyr1131) Set (#171-V50009M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-IGF-1R (Tyr1131) Set

171-V50009M
1 x 96-well, Includes coupled magnetic beads and detection antibodies for detecting phosphorylated IGF-1R (Tyr1131), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-IR-β (Tyr1146) Set

171-V50031M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated IR-β (Tyr1146), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-IRS-1 (Ser636/Ser639) Set

171-V50030M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated IRS-1 (Ser636/Ser639), requires reagent kit and lysates

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Bio-Plex Pro Phospho-I?B-a (Ser32/Ser36) Set (#171-V50010M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-IκB-α (Ser32/Ser36) Set

171-V50010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated IκB-α (Ser32/Ser36), requires reagent kit and lysates

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Bio-Plex Pro Phospho-JNK (Thr183/Tyr185) Set (#171-V50011M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-JNK (Thr183/Tyr185) Set

171-V50011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated JNK (Thr183/Tyr185), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-Lyn (Tyr507) Set

171-V50032M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Lyn (Tyr507), requires reagent kit and lysates

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Bio-Plex Pro Phospho-MEK1 (Ser217/Ser221) Set (#171-V50012M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-MEK1 (Ser217/Ser221) Set

171-V50012M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated MEK1 (Ser217/Ser221), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-mTOR (Ser2448) Set

171-V50033M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated mTOR (Ser2448), requires reagent kit and lysates

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Bio-Plex Pro Phospho-NF-?B p65 (Ser536) Set (#171-V50013M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-NF-κB p65 (Ser536) Set

171-V50013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated NF-κB p65 (Ser536), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p38 MAPK (Thr180/Tyr182) Set (#171-V50014M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p38 MAPK (Thr180/Tyr182) Set

171-V50014M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p38 MAPK (Thr180/Tyr182), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-p53 (Ser15) Set

171-V50034M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p53 (Ser15), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p70 S6 Kinase (Thr421/Ser424) Set (#171-V50015M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p70 S6 Kinase (Thr421/Ser424) Set

171-V50015M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p70 S6 kinase (Thr421/Ser424), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p70 S6 Kinase (Thr389) Set (#171-V50016M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p70 S6 Kinase (Thr389) Set

171-V50016M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p70 S6 kinase (Thr389), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-p90 RSK (Ser380) Set

171-V50035M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p90 RSK (Ser380), requires reagent kit and lysates

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Bio-Plex Pro Phospho-PDGFR-a (Tyr754) Set (#171-V50017M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-PDGFR-α (Tyr754) Set

171-V50017M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PDGFR-α (Tyr754), requires reagent kit and lysates

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Bio-Plex Pro Phospho-PDGFR-ß (Tyr751) Set (#171-V50018M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-PDGFR-β (Tyr751) Set

171-V50018M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PDGFR-β (Tyr751), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-PI3K p85 (Tyr458) Set

171-V50036M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PI3K p85 (Tyr458), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-PTEN (Ser380) Set

171-V50037M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PTEN (Ser380), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-S6 Ribosomal Protein (Ser235/Ser236) Set

171-V50038M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated S6 ribosomal protein (Ser235/Ser236), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Smad2 (Ser465/Ser467) Set (#171-V50019M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Smad2 (Ser465/Ser467) Set

171-V50019M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Smad2 (Ser465/Ser467), requires reagent kit and lysates

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Bio-Plex Pro™ Phospho-Src (Tyr416) Set

171-V50039M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Src (Tyr416), requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Phospho-Stat1 (Tyr701) Set (#171-V50020M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat1 (Tyr701) Set

171-V50020M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat1 (Tyr701), requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Phospho-Stat3 (Ser727) Set (#171-V50021M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat3 (Ser727) Set

171-V50021M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat3 (Ser727), requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Phospho-Stat3 (Tyr705) Set (#171-V50022M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat3 (Tyr705) Set

171-V50022M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat3 (Tyr705), requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Phospho-Syk (Tyr352) Set

171-V50040M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Syk (Tyr352), requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Phospho-VEGFR-2 (Tyr1175) Set (#171-V50023M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-VEGFR-2 (Tyr1175) Set

171-V50023M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated VEGFR-2 (Tyr1175), requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Phospho-ZAP-70 (Tyr319) Set

171-V50041M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated ZAP-70 (Tyr319), requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total Akt Set (#171-V60001M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Akt Set

171-V60001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Akt, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total Btk Set

171-V60012M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Btk, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total c-Jun Set (#171-V60002M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total c-Jun Set

171-V60002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total c-Jun, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total CREB Set

171-V60013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total CREB, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total Erk1/2 Set (#171-V60003M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Erk1/2 Set

171-V60003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Erk1/2, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Total GSK-3ß Set (#171-V60004M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total GSK-3β Set

171-V60004M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total GSK-3β, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Total HER-2 Set (#171-V60005M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total HER-2 Set

171-V60005M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total HER-2, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total IGF-1R Set

171-V60014M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total IGF-1R, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total I?B-a Set (#171-V60006M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total IκB-α Set

171-V60006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total IκB-α, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Total JNK Set (#171-V60007M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total JNK Set

171-V60007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total JNK, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Total MEK1 Set (#171-V60008M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total MEK1 Set

171-V60008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total MEK1, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total mTOR Set

171-V60015M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total mTOR, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total p38 MAPK Set (#171-V60009M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total p38 MAPK Set

171-V60009M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total p38 MAPK, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro Total p70 S6 Kinase Set (#171-V60010M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total p70 S6 Kinase Set

171-V60010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total p70 S6 kinase, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total PTEN Set

171-V60016M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total PTEN, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Total Smad2 Set (#171-V60011M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Smad2 Set

171-V60011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Smad2, requires reagent kit and lysates

List Price:   $281.00
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Bio-Plex Pro™ Total Src Set

171-V60017M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Src, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro™ Total ZAP-70 Set

171-V60018M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total ZAP-70, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro™ Total Human GADPH Set

171-V60019M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total human GADPH, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro™ Total β-Actin Set

171-V60020M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total β-actin, requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro EGF-Treated HEK293 Lysate (#171-YZ0001) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated HEK-293 Lysate

171-YZ0001
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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EGF-Treated HeLa Lysate (#171-YZ0002) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated HeLa Lysate

171-YZ0002
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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EGF-Treated SK-Br3 Lysate (#171-YZ0003) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated SK-Br3 Lysate

171-YZ0003
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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IFN-a-Treated HeLa Lysate (#171-YZ0004) - Bio-Plex Pro Cell Signaling Assay
IFN-α-Treated HeLa Lysate

171-YZ0004
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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IGF-1-Treated HEK-293 Lysate (#171-YZ0005) - Bio-Plex Pro Cell Signaling Assay
IGF-1-Treated HEK-293 Lysate

171-YZ0005
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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NGFß-Treated PC12 Lysate (#171-YZ0006) - Bio-Plex Pro Cell Signaling Assay
NGFβ-Treated PC12 Lysate

171-YZ0006
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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PDGF-Treated NIH3T3 Lysate (#171-YZ0007) - Bio-Plex Pro Cell Signaling Assay
PDGF-Treated NIH3T3 Lysate

171-YZ0007
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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TNF-a-Treated HeLa Lysate (#171-YZ0008) - Bio-Plex Pro Cell Signaling Assay
TNF-α-Treated HeLa Lysate

171-YZ0008
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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UV-Treated HEK-293 Lysate (#171-YZ0009) - Bio-Plex Pro Cell Signaling Assay
UV-Treated HEK-293 Lysate

171-YZ0009
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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VEGF-Treated HUVEC Lysate (#171-YZ0010) - Bio-Plex Pro Cell Signaling Assay
VEGF-Treated HUVEC Lysate

171-YZ0010
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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H2O2-Treated Ramos

171-YZ0011
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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H2O2-Treated Jurkat

171-YZ0012
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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Src-Transfected NIH3T3 Lysate

171-YZ0013
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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Phosphatase-Treated HeLa Lysate (#171-YZB001) - Bio-Plex Pro Cell Signaling Assay
Phosphatase-Treated HeLa Lysate

171-YZB001
Pkg of 1, lyophilized cell lysate, negative control for phosphoprotein detection methods

List Price:   $49.00
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Untreated HEK-293 (#171-YZT001) - Bio-Plex Pro Cell Signaling Assay
Untreated HEK-293

171-YZT001
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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Untreated HeLa Lysate (#171-YZT002) - Bio-Plex Pro Cell Signaling Assay
Untreated HeLa Lysate

171-YZT002
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

List Price:   $49.00
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Untreated K-562
Untreated K-562 lysate

171-YZT003
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Number Description Options
6285 Bio-Plex Pro Cell Signaling Assays Product Information Sheet, Rev C Click to download [ Add to Cart (Free) ]
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download
10024929 Instruction Manual, Bio-Plex Pro Cell Signaling Assays, Rev C Click to download
10024930 Instruction Manual, Bio-Plex Pro Assays, Quick Guide 3, Rev C Click to download
6330 Profiling of Cell Signaling Pathways and Angiogenesis Biomarkers in Human Cancer Tissue Click to download
6331 Validation and Application of Bio-Plex Pro Cell Signaling Assays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download
6504 Applications of Bio-Plex Pro Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways, Rev A Click to download