Bio-Plex Pro Magnetic Cell Signaling Assays

Bio-Plex Pro Magnetic Cell Signaling Assays

Overview

Bio-Plex Pro cell signaling assays are magnetic bead–based immunoassays for the detection of intracellular phosphoproteins and total target proteins in cell and tissue lysates.

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Description

Description

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Bio-Plex Assay Ordering Options

Multiplex Assay Kits Buy Digital PCR Assays

Select complete kits for multiplex target panels

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Singleplex Assays Buy Digital PCR Assays

Select individual target analytes of interest.

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Bio-Plex Pro Magnetic Cell Signaling Assays are multiplexed immunoassays for the detection of intracellular phosphoproteins in cell and tissue lysatesd using only 50 µl of lysate.

Features and Benefits of Magnetic Cell Signaling Assays

  • Assay protocol optimized for high sensitivity and broad dynamic range
  • Highly specific antibodies developed and validated by Cell Signaling Technology, Inc.
  • Higher specificity sandwich format that minimizes cross-reactivity with other cellular proteins
  • Low sample volume requirement
  • Minimal hands-on time
  • Assay quick guide to get you started right away

Applications and Uses of Magnetic Cell Signaling Assays

The capacity for multiplexed biomarker analysis makes the Bio-Plex Multiplex Immunoassay System the ideal platform for signal transduction assays. Bio-Plex Pro Magnetic Cell Signaling Assays can be used to determine intracellular levels of phosphorylated proteins in the investigation of diverse biological processes including apoptosis, translation control, gene expression, cell cycle regulation, cytoskeletal restructuring, and neurological function.

Required Reagents

No additional reagents are needed when purchasing a preconfigured Bio-Plex Pro Cell Signaling Panel. your Bio-Plex Pro cell signaling phosphoprotein or total target assay of interest.

Instrument Compatibility

Compatible with these readers and wash stations:

More Information

Other Bio-Plex magnetic bead–based immunoassays are available for diverse fields of research:

Learn more about the applications of multiplexing immunoassays.

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Specifications

Specifications

Reactive species
Human, mouse, rat
Matrices
Cell culture lysate, tissue lysate
Precision
Intra-assay CV <15%
Inter-assay CV <25%

Values vary by assay.

Ordering

Ordering

items

Use the filters below to refine results!

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LQ00000S6KL81S

1 x 96-well plate, includes coupled magnetic beads and detection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals for the detection of 9 MAPK pathway markers

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LQ00006JK0K0RR

1 x 96-well plate, includes coupled magnetic beads and detection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals for the detection of 8 Akt pathway markers

Accessories

Accessories

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(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included
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30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles
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171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays

Kits & Reagents

Kits & Reagents

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171304006M
1 x 96-well, includes cell lysis buffer, cell lysis factor QG, cell wash buffer, bead resuspension buffer, detection antibody diluent, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions
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171304515
330 ml, for 1 x 96-well assay, for use with Bio-Plex Pro cell signaling assays only, compatible with both magnetic and vacuum separation methods
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171YZ0003

Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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171YZ0004

Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Bio-Plex-Pro-NGFb-Treated-PC12-Lysate-SKU-171-YZ0006-Cell-Signaling-Assay.jpg
171YZ0006

Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Bio-Plex-Pro-PDGF-Treated-NIH3T3-Lysate-SKU-171-YZ0007-Cell-Signaling-Assay.jpg
171YZ0007

Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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171YZ0009

Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

Software

Software

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171001510
Bio-Plex Data Pro™ software (5 seats), for multi-experiment analysis and advanced data visualization, and Bio-Plex Manager™ software (5 seats), for instrument data evaluation and optimization. CDs and security HASP key included
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12015390
Bio-Plex Data Pro Plus Software for multi-experiment analysis and advanced data visualization, sharing, and analysis

Singleplexes

Singleplexes

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171V60019M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total human GAPDH, requires reagent kit and lysates

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171V60020M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total β-actin, requires reagent kit and lysates

Documents

Documents

Number Description Options
10024929
Instruction Manual, Bio-Plex Pro Cell Signaling Assays, Rev C
10024930
Instruction Manual, Bio-Plex Pro Assays, Quick Guide 3, Rev C
6331
Validation and Application of Bio-Plex Pro Cell Signaling Assays
6285
Bio-Plex Pro Cell Signaling Assays Product Information Sheet, Rev C
6504
Applications of Bio-Plex™ Pro Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways, Rev A
6335
Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide
5297
Bio-Plex Pro™ Assay Reference Guide, Ver D
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
3235
Comparison of Bio-Plex Pro Assay Performance Using the Bio-Plex 200 and Luminex xMAP INTELLIFLEX Systems