Bio-Plex Pro™ Human Isotyping Assays

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Overview

Bio-Plex Pro™ Human Isotyping Assays — Performance Characteristics
Parameter Characteristic
Reactive species Human
Intra-assay precision (%CV) ≤10%
Inter-assay precision (%CV) ≤15%
Accuracy (% recovery) 80–120%
Dilution linearity (R2) >0.99
Compatible sample matrices Serum, plasma, culture media
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Bio-Plex® Technology and Applications

The Bio-Plex Pro human isotyping immunoassays are available in a multiplex format and offer robust and reproducible measurement of eight different immunoglobulin subclasses or isotypes associated with

  • Allergic response
  • Immunodeficiency disorders
  • Autoimmune diseases
  • Cancer
  • Infectious diseases
  • Drug and vaccine development

Bio-Plex Pro human isotyping immunoassays are available in all-in-one kit configurations that include multiplex and singleplex kits.

  • lgG1
  • lgG2
  • lgG3
  • lgG4
  • lgG total
  • lgA
  • lgM
  • lgE

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • All-in-one kit format for both multiplex and singleplex assays
  • One diluent optimized for use with samples, standards, and controls
  • Assay quick guide to get you started right away
  • 1-level quality control

Assay Formats Available

  • All-in-one multiplex kit — for detecting human IgG1, IgG2, IgG3, IgG4, IgA, and IgM
  • All-in-one singleplex kits — for detecting human IgA, IgM, IgE, and IgG total

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Bio-Plex Pro Human Isotyping Panel, 6-plex
Bio-Plex Pro™ Human Isotyping Panel, 6-plex

171-A3100M
1 x 96-well plate, includes coupled magnetic beads, detection antibodies, standards, controls, and reagents for detecting human IgG1, IgG2, IgG3, IgG4, IgA, and IgM, all-in-one kit

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Bio-Plex Pro Human IgG Total Isotyping Assay
Bio-Plex Pro™ Human IgG Total Isotyping Assay

171-A3103M
1 x 96-well plate, includes coupled magnetic beads, detection antibodies, standards, controls, and reagents for detecting human IgG1, IgG2, IgG3, and IgG4, all-in-one kit

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Bio-Plex Pro Human IgA Isotyping Assay
Bio-Plex Pro™ Human IgA Isotyping Assay

171-A3101M
1 x 96-well plate, includes coupled magnetic beads, detection antibodies, standards, controls, and reagents for detecting human IgA, all-in-one kit

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Bio-Plex Pro Human IgE Isotyping Assay
Bio-Plex Pro™ Human IgE Isotyping Assay

171-A3102M
1 x 96-well plate, includes coupled magnetic beads, detection antibodies, standards, controls, and reagents for detecting human IgE, all-in-one kit

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Bio-Plex Pro Human IgM Isotyping Assay
Bio-Plex Pro™ Human IgM Isotyping Assay

171-A3104M
1 x 96-well plate, includes coupled magnetic beads, detection antibodies, standards, controls, and reagents for detecting human IgM, all-in-one kit

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Number Description Options
6344 Bio-Plex Pro™ Human Isotyping Panel, Rev B Click to download
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
10028370 Instruction Manual, Bio-Plex Pro Human Isotyping Assays, Rev B Click to download
10028208 Instruction Manual, Bio Plex Pro Assays Quick Guide 6, Rev A Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6394 Development and Validation of a Newly Reconfigured Bio-Plex Pro Human Isotyping Assay Panel, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download