Bio-Plex Pro Human Angiogenesis Multiplex Assays

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Overview

Specifications
Intra-assay CV ≤15%  
Inter-assay CV ≤25%  
Accuracy (% recovery) 70–130%  
Cross-reactivity Negligible  
Matrices Cell culture, serum, plasma  
 
Representative Working Assay Range and Sensitivity
Targets Range Sensitivity
Angiopoietin-2 500–32,000 pg/ml ≤50 pg/ml
Follistatin 100–32,000 pg/ml ≤15 pg/ml
G-CSF 2–32,000 pg/ml ≤10 pg/ml
HGF 100–32,000 pg/ml ≤30 pg/ml
IL-8 2–32,000 pg/ml ≤2 pg/ml
Leptin 100–32,000 pg/ml ≤50 pg/ml
PDGF-BB 100–32,000 pg/ml ≤7 pg/ml
PECAM-1 1,000–32,000 pg/ml ≤120 pg/ml
VEGF 2–32,000 pg/ml ≤10 pg/ml
Available Bio-Plex Pro Disease Research Assays
Precision Pro™ Human Cytokines Human Isotyping
Human Diabetes
Human Acute Phase Human Angiogenesis
IL-1β
IgG1
Adiponectin
α-2-macroglobulin
Angiopoeitin-2
IL-2
IgG2
Adipsin
CRP
Follistatin
IL-4
IgG3
C-peptide
Ferritin
G-CSF
IL-5
IgG4
Ghrelin
Fibrinogen
HGF
IL-6
IgM
GIP
Haptoglobin
IL-8
IL-10
IgA
GLP-1
Procalcitonin
Leptin
IL-12 (p70)
IgE
Glucagon
SAA
PDGF-BB
IL-1β
 
IL-6
SAP
PECAM-1
IFN-γ
 
Insulin
Tissue plasminogen activator
VEGF
TNF-α
 
Leptin
 
   
PAI-1
   
   
Resistin
   
   
TNF-α
   
   
Visfatin
   
What You Need For Bio-Plex Pro Disease Research Assays
What is Needed for Performing Bio-Plex Pro Assay

Bio-Plex Pro assay panel — 1 x 96-well and 10 x 96-well, includes coupled magnetic beads, detection antibodies, standards, and controls.

Bio-Plex Pro reagent kit — includes assay buffer, wash buffer, streptavidin-PE, 96-well filter plate, sealing tape, and panel-specific instructions required to prepare assays and aquire data.

Bio-Plex diluent kit — choose the appropriate diluent kit for the panel for diluting samples, standards, and controls.

Using Bio-Plex® Technology for Disease Research

Angiogenesis research involves the measurement and monitoring of a host of protein targets that either induce or suppress the growth of blood vessels. Many of these targets can be measured by the Bio-Plex Pro™ human angiogenesis multiplex assay, allowing the detection of nine human angiogenesis biomarkers in just 3 hr with as little as 13 µl of sample, using the Bio-Plex® 200 system. The assay is available as a 9-plex panel (alone or as a complete kit inluding all necessary reagents) and is validated for detection in human samples. Targets detected by the Bio-Plex Pro human angiogenesis multiplex assay include the following:

  • angiopoietin-2
  • follistatin
  • G-CSF
  • HGF
  • IL-8
  • leptin
  • PDGF-BB
  • PECAM-1
  • VEGF

Based on 8.0 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Allows detection of 9 human angiogenesis biomarkers
  • Provides excellent sensitivity with both the automated washing system and a standard vacuum manifold
  • Eliminates manual filtration steps (with automated washing), increasing throughput and reproducibility
  • Includes controls for generating QC samples

Additional Neccessary Reagents

If a complete kit is purchased, no additional reagents are required. If the 9-plex panel is purchased alone, the following additional items are needed to perform a Bio-Plex Pro human angiogenesis assay:

  • Bio-Plex Pro angiogenesis reagent kit — includes assay reagents and instructions required to prepare samples and acquire data
  • Bio-Plex Pro human serum diluent kit — includes diluents for diluting samples, standards, and controls

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Species Human
Immunoassay for Angiogenesis
Bead type Magnetic
Format Premixed panel
Custom assay options Not available
Compatible with Bio-Plex® 200 system
Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
10010584 Bio-Plex Pro Angiogenesis Instruction Manual, Rev A Click to download
5653 Bio-Plex Pro Human Angiogenesis Assay Panel Product Sheet, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download