Bio-Plex Pro™ Rat Diabetes Assays

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Overview

Bio-Plex Pro™ Rat Diabetes Assays

Singleplex Sets
Bead Regions
Ghrelin
64
GLP-1
62
Glucagon
63
Leptin
65
PAI-1
61
Specifications for the NEW Bio-Plex Pro™ Rat Diabetes Assays

Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for <20% intra-assay CV, <30% inter-assay CV, and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.


Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml Assay Precision
Targets
LLOQ
ULOQ
LOD
Intra-assay %CV Inter-assay %CV
Ghrelin
1
 
16,152
 
0.3
  4 4
GLP-1
4
 
6,062
 
3
  8 6
Glucagon
6
 
4,443
 
6
  3 4
Leptin
24
 
130,465
 
5
  4 3
PAI-1
49
 
66,888
 
27
  5 3

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

Bio-Plex® Technology and Applications

To create a Bio-Plex® custom assay, click Launch Assay Builder.

Launch Assay Builder

Bio-Plex Pro™ rat diabetes assays are magnetic bead–based assays designed to measure five obesity biomarkers, using as little as 12.5 µl of sample per well. These assays are compatible with the new Bio-Plex Pro rat cytokine, chemokine, and growth factor assays for an expanded biomarker profile. They have been developed to deliver accurate, reproducible measurements with complete flexibility to meet your research needs.

Key Features and Benefits

  • Quality — designed for enhanced assay sensitivity and extended assay working ranges
  • Robustness — tested in serum and plasma sample matrices
  • Increased productivity — measure multiple markers simultaneously in approximately 4 hr
  • Option to use a magnetic wash station — take advantage of the magnetic workflow for simplified assay preparation and realize consistently improved reproducibility (also compatible with the vacuum wash station)

Flexible Ordering Options

  • Singleplex sets — when you want to test for a single analyte
  • Customized Express assays — use the Bio-Plex Assay Builder to select your analytes of interest; please allow an extra 12 days for custom mixing and quality control testing

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Incubation time, hr 1
Sample volume, µl 12.5
Accuracy (recovery range) 80–120%
Precision  
      Intra-assay% CV <20%
      Intra-assay% CV <30%
Bio-Plex Pro Rat Metabolic Ghrelin Set
Bio-Plex Pro™ Rat Diabetes Ghrelin Set

171-L7001M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat ghrelin, requires reagent kit (#171-304070) and standards, which are not included

List Price:   $251.00
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Bio-Plex Pro Rat Diabetes GLP-1 Set
Bio-Plex Pro™ Rat Diabetes GLP-1 Set

171-L7003M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat GLP-1, requires reagent kit (#171-304070) and standards, which are not included

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Bio-Plex Pro Rat Diabetes Glucagon Set
Bio-Plex Pro™ Rat Diabetes Glucagon Set

171-L7004M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat glucagon, requires reagent kit (#171-304070) and standards, which are not included

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Bio-Plex Pro Rat Metabolic Leptin Set
Bio-Plex Pro™ Rat Diabetes Leptin Set

171-L7006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat leptin, requires reagent kit (#171-304070) and standards, which are not included

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Bio-Plex Pro™ Rat Diabetes PAI-1 Set
(Discontinued) Bio-Plex Pro™ Rat Diabetes PAI-1 Set

171-L7007M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat PAI-1, requires reagent kit (#171-304070) and standards, which are not included

This product is no longer available. Please contact customer service for more information on replacement product.
Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev C Click to download
6119 Multiplex Analysis Using Bio-Plex Pro Rat Cytokine and Diabetes Assays, Rev A Click to download [ Add to Cart (Free) ]
10010747 Instruction Manual, Bio-Plex Pro Diabetes Assays, Rev D Click to download
6342 Bio-Plex Pro Diabetes Assay Panels Product Information Sheet, Rev A Click to download
10024973 Bio-Plex Pro Assays Quick Guide 2, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download