Bio-Plex® MAGPIX™ Multiplex Reader



Parameters Bio-Plex® MAGPIX™ Multiplex Reader

Read time (96-well plate) ~ 60 min
Plate compatibilities 96-well only
Number of measurable analytes/well 50
Assay compatibility Magnetic bead–based assays
Acquisition software xPONENT 4.2
Analysis software Bio-Plex Manager™ software
Bio-Plex Data Pro™ software
(W x D x H)
16.5 x 60 x 43 cm
(6.5 x 23.5 x 17")
Frequently Asked Questions

1. Can the same beads for the Bio-Plex® MAGPIX™ system be used for the Bio-Plex® 100/200 systems?

The Bio-Plex MAGPIX system uses only 6.5 µm MagPlex magnetic beads and utilizes only 50 regions of the 100 bead maps employed on the Bio-Plex 100/200 systems. The regions highlighted in yellow are compatible with the Bio-Plex MAGPIX reader.

2. What software version is compatible with the Bio-Plex MAGPIX system?

The Bio-Plex MAGPIX system uses xPONENT software version 4.2 to acquire data and Bio-Plex Manager™ software for data review and optimization. After acquiring data with xPONENT, results can be automatically opened with Bio-Plex Manager for data review and optimization. Bio-Plex Data Pro™ software can then be used to combine multiple runs, to perform data organization, and for visualization to help discern differences in data groups quickly.

3. Are the calibration and verification beads for the Bio-Plex 100/200 systems and the Bio-Plex MAGPIX system the same?

No, the Bio-Plex MAGPIX system uses different calibration and verification beads than the Bio-Plex 100/200 systems.

4. How often must I perform calibration with the Bio-Plex MAGPIX system?

It is recommended to perform calibration once a week and performance verification every day — you can choose both options when “System Initialization” is selected. The system should be initialized every time

  • The system is moved
  • The temperature changes by more than 5ºC
  • Sample acquisition problems arise

5. Must I remeasure the probe height prior to each plate reading if the same type of plate is used?

No, once the probe height is adjusted for a particular plate type, the adjustment can be saved. If a new plate type is used, the instrument must be calibrated (use metal disk) to acquire its probe height. It is recommended to calibrate probe height whenever the probe is replaced for cleaning or when there are data acquisition problems.

6. What are the reasons for calibration or verification failure of MAGPIX instrument?

1. Calibration and verification beads were not fully suspended.
2. Wrong lot number or wrong target values were entered.
3. Calibration or verification beads were in the wrong well.
4. Fewer than 5 drops of calibration or verification beads were used.
5. Lots are expired.
6. The sample probe height is incorrect or it is clogged.
7. There is a clog in the probe or within the instrument.
8. The recommended drive fluid was not used.
9. The temperature and pressure are not within normal range.
10. There are error messages.

If all of the above are fine, perform the Clog Troubleshooting Procedure and attempt to recalibrate. If the system still fails, request to see the Control Trend Report for additional information. Verification failures can be due to carryover problems from a previous calibration. Performing four rinse cycles and two Clean commands using a 0.1 N sodium hydroxide solution.

7. How do I add new kit lots/values?

A CD containing all the relevant information is included in each kit. This CD is unique to each lot of the kit and is included with both the Calibration and Performance Kits.

8. Do I need to create a protocol before I create the batch?

You may create either the batch or the protocol first. In Batch setup, protocols and batches can be created simultaneously but can be saved separately to preserve experimental setup for later use.

9. What are the expected read times for a 96-well plate on the Bio-Plex MAGPIX system?

For a 96-well plate, the read time is approximately 50–60 min.

10. Can Bio-Plex assays be run on the Bio-Plex MAGPIX system?

Bio-Plex Pro assays that use MagPlex beads can be run on the Bio-Plex MAGPIX system; refer to the assay instruction manual for correct bead regions and bead type. Bio-Plex assays that use the Bio-Rad 8.0 µm magnetic beads or Luminex 5.6 µm polystyrene beads cannot be run on the MAGPIX system.


Bio-Plex Pro™ human cancer biomarker panel assays
Bio-Plex Pro human Th17 assays
Bio-Plex Pro mouse Th17 assays
Bio-Plex Pro human cytokine assays
Bio-Plex Pro mouse cytokine assays
Bio-Plex Pro rat cytokine assays
Bio-Plex Pro human diabetes assays
Bio-Plex Pro mouse diabetes assays
Bio-Plex Pro rat diabetes assays
Bio-Plex Pro TGF-β assays

Bio-Plex Pro magnetic assays compatible with the Bio-Plex MAGPIX multiplex reader.

11. Is there a hardware protection key included with the Bio-Plex MAGPIX system?

You must enter an alpha-numeric code to activate xPONENT software. Bio-Plex Manager does require a hardware protection key.

12. Most of my samples are very dirty. Can I set up a more thorough maintenance routine to clean the system?

Yes, after running the plate, you can set up custom maintenance routines: go to the Maintenance tab, select the button for Cmds & Routines, enter the desired steps, and save the protocol. We recommend Rinse 3X, Alcohol flush 3X, and Wash 3X. This will then be available in the Cmds & Routines window.

13. How do I know that the sample probe is clogged?

The following problems are an indication that the sample probe may be clogged:

  • MFI values are unusual
  • Low bead count
  • Failing Calibration/Verification

To rectify the problem:

  • Remove probe and sonicate narrow end for 1–2 min
  • Flush the probe from the narrow end to the thicker end with diH2O
  • Replace the probe and run the Clog Removal routine
    • Alcohol flush
    • Wash
    • Clean
    • Wash

14. What are the guidelines for preventative maintenance?


  • Check fluid levels — this can be seen by looking behind the front door where the fluid bottles are found. One bottle of Drive Fluid runs 8 full plates
  • During period of use, run a Daily Startup, which is a Rinse command — this acts as a Prime and also uses drive fluid, and alcohol flush, followed by 2 rinses
  • Sanitize the lines using 10%–20% household bleach
  • Run a shutdown


Replace the alcohol in the alcohol well of the off-plate reagent block with a 0.1 N sodium hydroxide solution. Perform a Clog Removal routine:

  • Remove and clean the sample probe
  • Perform visual inspection — look for any leaks or precipitated drive fluid
  • Calibrate and verify

Six Months

  • Clean air filters
  • Replace the syringe seal on the syringe pump

Twelve Months

  • Replace the sample probe tube
  • Replace the sheath filter

15. Can I set up the system to automatically export data to Bio-Plex Manager software for analysis?

Yes, the system can be set up to automatically create a Bio-Plex Manager RBX file and then automatically open the data in Bio-Plex Manager software for ease of analysis.

16. Since the Bio-Plex MAGPIX system has one setting, can I run Bio-Plex assays developed on a high PMT setting?

Yes, Bio-Plex Pro assays developed on a high PMT setting can be run on the Bio-Plex MAGPIX system. During the course of an experimental study, only one instrument platform should be used (either the Bio-Plex MAGPIX or Bio-Plex 100/200 systems).


Sample Concentrations Using Bio-Plex® 200 and Bio-Plex® MAGPIX™ Systems


Sample concentrations obtained using the Bio-Plex 200 () and Bio-Plex MAGPIX systems ().


Standard Curves with Unknown Concentrations

Standard curve comparison when the Bio-Plex Pro™ human cytokine 27-plex panel is run on the laser-based Bio-Plex 200 system () and the LED-based Bio-Plex MAGPIX system (). Representative curves are shown in a log-log scale.

Comparison of Flow Cytometry-Based and LED/Image-Based Systems

Flow Cytometry–Based Analysis — Traditional xMAP Technology (Bio-Plex 200, Bio-Plex 3D Systems)

LED/Image-Based Analysis — New xMAP Technology (Bio-Plex MAGPIX System)


Comparison of a traditional flow cytometry–based system with an LED/image-based system.


Bio-Plex Assay Builder

Use the Bio-Plex® Assay Builder to design your customized Bio-Plex Assay in four easy steps

With Assay Builder you can also:

  • Get a price quote
  • Place your order online
  • Look up an existing assay by catalog number

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder

The Bio-Plex MAGPIX multiplex reader is a compact, robust system for magnetic bead–based immunoassays. This multiplex reader is capable of reading assays designed on magnetic xMAP (MagPlex) beads, compatible with Bio-Plex Pro™ magnetic assays.

Key Features of the Bio-Plex MAGPIX Multiplex Reader

  • Get more data by reading up to 50 analytes per sample
  • Simple and convenient ELISA-like workflow
  • Benefit from sample and cost savings with Bio-Plex Pro magnetic bead–based assays in single or multiplex format
  • Provide improved multiplex productivity and convenience with magnetic bead–based assays
  • Save bench space with a compact footprint
  • Conserve precious resources with an affordable low maintenance system
  • Obtain the same quality results achieved on the Bio-Plex® 200 or Bio-Plex 3D systems

Take advantage of automated instrument management and other software features:

  • Automatic loading of recommended start-of-day maintenance routines based on current instrument status
  • Performance monitoring during data acquisition alerts the user to performance issues and loads recommended maintenance to resolve issues
  • Simple step-wise troubleshooting

Benefits of Bio-Plex Manager MP Software

  • Detection and resolution of performance issues ensures data quality
  • Confidence in proper instrument maintenance leads to confidence in results
  • Highest quality results maximize the value of your experiments
  • Simple integration with Bio-Plex analysis software to quickly assemble and understand your data
  • Simple interface and built-in guidance enable a user of any experience level to easily run the Bio-Plex MAGPIX multiplex reader

Components of the Bio-Plex MAGPIX Multiplex Reader

  • Bio-Plex MAGPIX instrument
  • Bio-Plex Manager MP software
  • Bio-Plex Manager software
  • PC with xPONENT 4.2 acquisition software installed
  • Probe height adjustment plate
  • Calibration kit
  • Verification kit
  • 2 drive fluid cartridges
  • 2 waste containers
  • Getting started guide
  • Upgrade quick guide

Related Products

More Information
To learn more about Bio-Plex MAGPIX multiplex reader, refer to the documents listed below:

Visit the Bio-Plex software resource page for the latest software updates, information, and standard lot values.


Parameters Bio-Plex MAGPIX System
Read time (1 x 96-well plate) ~ 60 minutes
Plate compatibilities 96-well only
Number of measurable analytes / well 50
Assay compatibility Magnetic bead–based assays
Acquisition software Bio-Plex Manager MP
Analysis software Bio-Plex Manager™ software
Bio-Plex Data Pro™ software
Dimensions (W x D x H) 16.5 x 60 x 43 cm
(6.5 x 23.5 x 17")

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Bio-Plex® MAGPIX™ Multiplex Reader with Bio-Plex Manager™ MP Software

Includes Bio-Plex MAGPIX instrument and accessories, PC, Excel, Bio-Plex Manager MP software, Bio-Plex Manager, probe height adjustment plate, and Getting Started Guide

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Bio-Plex Manager™ MP Software Upgrade

Includes Bio-Plex Manager MP software, Bio-Plex Manager 6.1 software, probe height adjustment plate, Getting Started Guide, and Upgrade Quick Guide

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Number Description Options
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
6006 Bio-Plex MAGPIX, Bio-Plex 200, and Bio-Plex 3D Systems – Comparable Performance Across the Board, Product Information Sheet, Rev A Click to download
6005 Bio-Plex™ MAGPIX Multiplex Reader, Product Information Sheet, Rev C Click to download [ Add to Cart (Free) ]
6486 Bio-Plex Manager MP Software, Product Flyer, Rev A Click to download [ Add to Cart (Free) ]
10032257 Instruction Manual, Bio-Plex Manager MP Software, User Guide v. 1.0, Rev A Click to download
10032064 Instruction Manual, Bio-Plex Manager MP Software, Getting Started Guide v. 1.0, Rev A Click to download
171051222 Instruction Manual, Bio-Plex Manager MP Software Upgrade, Quick Guide v. 1.0, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download
6313 Overcoming the Cost and Performance Limitations of ELISA with Bio-Plex® Multiplex Readers, Rev A Click to download