Bio-Gel Hydroxyapatite HT and HTP Media

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Overview

Specifications
  Bio-Gel HTP Gel Bio-Gel HT Gel, DNA Grade Bio-Gel HTP Gel
Flow rate* (cm/hr) >35 >5 >25
BSA adsorbed** (mg per dry gram) 10 10 10
Calf thymus DNA adsorbed (mg per dry gram) >500 >800 >500
Hydrated volume 2–3 ml/g 2–3 ml/g

* Flow rate determined on a 1.5 x 10 cm column with 40 cm hydrostatic pressure.
** Batchwise uptake.

Bio-Gel HT and HTP Media

Hydroxyapatite (Ca5(PO4)3(OH)2 is a form of calcium phosphate that can be used for the separation or purification of proteins, enzymes, nucleic acids, viruses, and other macromolecules. Hydroxyapatite chromatography can be used at any stage, from initial capture to final polishing. Hydroxyapatite has unique selectivity and resolution, and often separates proteins that appear to be homogeneous by other chromatographic and electrophoretic techniques.

Bio-Gel crystalline hydroxyapatite is chemically and thermally stable, is compatible with a wide range of aqueous and organic solvents, and can be sanitized in NaOH. It has a pH tolerance of >5.5 and is autoclavable. Three types of media are available:

Bio-Gel HT gel— Ideal for the purification of proteins, nucleic acids, and other macromolecules, Bio-Gel HT is prepared by the method of Tiselius (1956). It is suspended in 10 mMsodium phosphate buffer containing 0.02% NaN3 and should be stored at 4°C.

Bio-Gel HTP gel— This is the material described by Tiselius, which has been dried by a unique process developed by Bio-Rad. It may be stored without refrigeration.When suspended in buffer, it has characteristics similar to Bio-Gel HT gel; 1 g hydrates to 2–3 ml.

DNA grade Bio-Gel HTP gel— A dry powder with a smaller particle size than HTP gel, this gel significantly increases its capacity and enhances its selectivity for double-stranded DNA. Due to its small particle size, it is recommended for batch mode chromatography or for use with very short columns.

Bio-Gel HT Hydroxyapatite

130-0150
250 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer

List Price:   $297.00
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Bio-Gel HT Hydroxyapatite

130-0151
500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer

List Price:   $512.00
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Bio-Gel HTP Hydroxyapatite

130-0420
100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel

List Price:   $322.00
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Bio-Gel HTP Hydroxyapatite

130-0421
1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel

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Bio-Gel HTP Hydroxyapatite

130-0425
5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel

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Bio-Gel HTP Hydroxyapatite

130-0520
100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA

List Price:   $336.00
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Number Description Options
LIT217 Instruction Manual, Bio-Gel HT, Bio-Gel HTP, DNA Grade Bio-Gel HTP, Hydroxyapatite, Rev C Click to download [ Add to Cart (Free) ]