Bio-Dot® and Bio-Dot SF Microfiltration Apparatus

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Overview

Specifications
Features Bio-Dot Bio-Dot SF
Format Dot blot Slot blot
Samples 96-well, 8 x 12 format 48-well, 6 x 8 format
Sample volume 50–600 µl 50–500 µl
Well size 3 mm diameter 7 x 0.75 mm
Quantitation with densitometer Yes, but Bio-Dot SF Unit Recommended Yes
Overnight incubations Yes No
Membrane size (W x L) 12 x 9 cm 12 x 9 cm
Dimensions (W x H x D) 9 x 6.5 x 12" 9.5 x 7 x 12"
Weight 1.1 kg (2.5 lb) 1.2 kg (2.6 lb)
Bio-Dot® Apparatus Assembly
Bio-Dot® SF Apparatus Assembly

The 96-well Bio-Dot® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Many experimental protocols can be accommodated by using interchangeable templates. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle, making quantitation by densitometry more reproducible. Both are available as a complete, independent unit, or as a modular template without the manifold base. Features include:

  • Resistance to 100% ethanol, strong acid, and NaOH
  • Autoclavability
  • Patented sealing gasket to eliminate lateral leakage
  • Easy sample application with microplate-based spacing
  • Flow valve (3-way) for adjustable vacuum
Bio-Dot Microfiltration System

170-3938
Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket support plates, gasket

List Price:   $995.00
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Bio-Dot Apparatus

170-6545
Microfiltration blotting device, includes 96-well sample template, vacuum manifold base, gasket support plate, gasket

List Price:   $627.00
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Bio-Dot Module

170-6547
Pkg of 1, 96-well sample template, modular unit converts Bio-Dot SF apparatus to Bio-Dot apparatus; for blot transfer microfiltration

List Price:   $473.00
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Bio-Dot SF Apparatus

170-6542
Slot blotting microfiltration device, includes 48-well slot-format sample template, vacuum manifold base, gasket support plate, gasket, filter paper

List Price:   $627.00
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Bio-Dot SF Module

170-6543
Pkg of 1, 48-well slot-format sample template, modular unit converts Bio-Dot apparatus to Bio-Dot SF apparatus; for slot blot transfer

List Price:   $462.00
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Bio-Dot Gaskets

170-6546
Pkg of 3, replacement gasket for Bio-Dot apparatus (#170-6545)

List Price:   $82.00
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Bio-Dot SF Gaskets

170-6544
Pkg of 2, replacement gasket for Bio-Dot SF apparatus (#170-6542)

List Price:   $63.00
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Bio-Dot/Bio-Dot SF Filter Paper #162-0161
Bio-Dot/Bio-Dot SF Filter Paper

162-0161
Pkg of 60, 11.3 x 7.7 cm, precut absorbent filter paper for blotting applications requiring thick, high-wet-strength filter paper

List Price:   $41.00
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Number Description Options
M1706542 Instruction Manual, Bio-Dot SF Microfiltration Apparatus, Rev C Click to download
M1706545 Instruction Manual, Bio-Dot Microfiltration Apparatus, Rev C Click to download