Auto-sealing lids are reusable, automation-friendly PCR plate sealers that prevent evaporation and contamination during reaction setup and seal tightly for PCR when the cycler lid is closed. When auto-sealing lids are used in combination with a Moto Alpha motorized cycler lid, automated plate sealing and unsealing can be accomplished without an expensive heat sealing or piercing device.
The lids are constructed of metal with an attached compressible pad, and they can be applied by robotic gripper devices. Three varieties are available: flat lid, arched lid, or arched lid with wide tabs. The lids can be reused up to 50 times.
- Flat lid — allows low-volume cycling (down to 1 µl) in 384-well plates using a Moto Alpha unit; remains tightly sealed to the PCR plate for up to 24 hours after cycling and can be used for short-term storage down to –20°C
- Arched lid — capable of self-release from the PCR plate after thermal cycling; can also be removed by robotic devices
- Arched lid with wide tabs — well suited for use with robotic grippers