Aurum™ Total RNA 96 Kit


The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

  • High-throughput total RNA isolation in less than 60 minutes
  • RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
  • Rapid silica binding-based purification technology in a 96-well plate format
  • Easy-to-use spin- or vacuum-compatible protocol
Format 96-well plate
Method Spin- or vacuum-mediated silica binding
Number of preps 192 (2 x 96-well plate)
Maximum starting material amounts 1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding Capacity >40 µg purified total RNA
Purity A260/A280 of 1.9–2.1
RNA size distribution >200 nucleotides
Preparation time <60 min (including DNase I digest)
Maximum elution volume 80 µl
Minimum elution volume 30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield** 5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells
*The elution volume is reduced to 30µl when
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.

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Aurum™ Total RNA 96 Kit

DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps

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Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
2919 Aurum Total RNA 96 Kit Product Information Sheet, Rev B Click to download
2925 Aurum Total RNA 96 Kit Protocol Overview Click to download
4110131 Instruction Manual, Aurum™ Total RNA 96 Kit, Rev C Click to download
6018 High-Throughput Automated Extraction of RNA Using the Aurum Total RNA 96 Kit, Rev A Click to download
6763 Gene Expression Real-Time PCR Workflow Flier, Ver A Click to download [ Add to Cart (Free) ]