Aurum™ Plasmid Mini Purification Module

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Aurum Plasmid Mini Purification Module

Overview

Lab Preparation Checklist
Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria.
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
Required Accessories Not Included in Kit:
95–100% lab grade ethanol  
Microcentrifuge capable of greater than 12,000 x g 1
Shaking water bath 1
Adjustable micropipets
   20–200 µl 1–12
   100–1000 µl 1–12
Pipet tips  
Recommended (Optional) Accessories:
Aurum vacuum manifold

DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
Aurum Plasmid Mini Purification Module
CS Module Honeycomb

Isolate plasmid DNA from transformed bacteria. Students inoculate minipreps with transformed bacterial colonies from their agar plates. They then isolate plasmid DNA using a three step alkaline lysis procedure followed by mini column chromatography. Purification can be carried out in microcentrifuges or using the Aurum vacuum manifold.

Once plasmid DNA is eluted, students verify insertion of the PCR product into the plasmid vector using restriction enzyme digestion analysis and agarose gel electrophoresis. Bgl II restriction enzyme, specific for screening the pJET1.2 plasmid, is supplied in the Ligation and Transformation module. Students can also determine plasmid DNA concentration using spectrophotometry or fluorometry in an optional step.

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Purify up to 20 µg of plasmid DNA rapidly and inexpensively without the use of toxic reagents or alcohol precipitations. Plasmid DNA is purified from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography. The highly purified plasmid DNA is free of salts, bacterial chromosomal DNA, and RNA, and is ideal for subsequent molecular biology–based applications such as automated sequencing, cloning, PCR, or restriction digestion analysis.

The Aurum Plasmid Mini Purification Module is an integral component of the Cloning and Sequencing Explorer Series. The module may also be purchased separately and used to isolate plasmid from bacteria containing any cloned gene.

Kit contains sufficient reagents to purify
DNA from 100 minicultures of plasmid-bearing
bacteria.
 
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
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Aurum Plasmid Mini Purification Kit

7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

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Plasmid Mini Purification Kit #732-6400EDU RepEx Aurum&trade; Plasmid Mini Purification Kit #732-6400EDU Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps 7326400, 7326400EDU, aurum, plasmid, mini, dna, purification, sample, preparation, columns, capless, collection, tubes, reagents, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>The seventh module in the Cloning and Sequencing Explorer Series, the Aurum&trade; Plasmid Mini Purification Module contains reagents to purify up to 20 &micro;g of DNA from a plasmid miniculture. The kit includes spin columns, collection tubes, buffers, and curriculum. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 732-6400EDU_0921121012543_en.html /webroot/web/html/lse/products/SKUs/autopopulated//732-6400EDU_0921121012543_en.html 1141 <b>Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria</b> &nbsp; 1 0 1580 DNA-binding columns 100 1 0 2700 Capless collection tubes, 2.0 ml 100 1 0 2373 Resuspension solution, 25 ml 1 1 0 2374 Lysis solution, 25 ml 1 1 0 2375 Neutralization solution, 40 ml 1 1 0 2701 Wash solution, 5x, 25 ml 1 1 0 2377 Elution solution, 16 ml 1 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&CartQuote=QT&quantity= false true false $217.14 true %24152.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&quantity= 7326400EDU
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(Discontinued) Aurum Vacuum Manifold

7326470EDU
Vacuum-mediated nucleic acid purification platform for spin columns or 96-well plates, includes column adaptor plate and all necessary accessories; education use only

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2664 Aurum Plasmid Mini Kit Product Information Sheet, Rev C Click to download
2671 Quick Guide, Aurum Vacuum Manifold Column Purification, Rev A Click to download
2663 Quick Guide, Aurum Plasmid Mini Kit Spin Format Protocol Overview, Rev A Click to download [ Add to Cart (Free) ]
4110111 Instruction Manual, Aurum Plasmid Mini Kit, Biotechnology Explorer, Rev C Click to download

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