Purify up to 20 µg of plasmid DNA rapidly and inexpensively without the use of toxic reagents or alcohol precipitations. Plasmid DNA is purified from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography. The highly purified plasmid DNA is free of salts, bacterial chromosomal DNA, and RNA, and is ideal for subsequent molecular biology–based applications such as automated sequencing, cloning, PCR, or restriction digestion analysis.
The Aurum Plasmid Mini Purification Module is an integral component of the Cloning and Sequencing Explorer Series. The module may also be purchased separately and used to isolate plasmid from bacteria containing any cloned gene.