Aurum™ Plasmid Mini Purification Module

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Aurum Plasmid Mini Purification Module

Overview

Lab Preparation Checklist
Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria.
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
Required Accessories Not Included in Kit:
95–100% lab grade ethanol  
Microcentrifuge capable of greater than 12,000 x g 1
Shaking water bath 1
Adjustable micropipets
   20–200 µl 1–12
   100–1000 µl 1–12
Pipet tips  
Recommended (Optional) Accessories:
Aurum vacuum manifold

DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
Aurum Plasmid Mini Purification Module
CS Module Honeycomb

Isolate plasmid DNA from transformed bacteria. Students inoculate minipreps with transformed bacterial colonies from their agar plates. They then isolate plasmid DNA using a three step alkaline lysis procedure followed by mini column chromatography. Purification can be carried out in microcentrifuges or using the Aurum vacuum manifold.

Once plasmid DNA is eluted, students verify insertion of the PCR product into the plasmid vector using restriction enzyme digestion analysis and agarose gel electrophoresis. Bgl II restriction enzyme, specific for screening the pJET1.2 plasmid, is supplied in the Ligation and Transformation module. Students can also determine plasmid DNA concentration using spectrophotometry or fluorometry in an optional step.

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Purify up to 20 µg of plasmid DNA rapidly and inexpensively without the use of toxic reagents or alcohol precipitations. Plasmid DNA is purified from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography. The highly purified plasmid DNA is free of salts, bacterial chromosomal DNA, and RNA, and is ideal for subsequent molecular biology–based applications such as automated sequencing, cloning, PCR, or restriction digestion analysis.

The Aurum Plasmid Mini Purification Module is an integral component of the Cloning and Sequencing Explorer Series. The module may also be purchased separately and used to isolate plasmid from bacteria containing any cloned gene.

Kit contains sufficient reagents to purify
DNA from 100 minicultures of plasmid-bearing
bacteria.
 
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
Number Description Options
2664 Aurum Plasmid Mini Kit Product Information Sheet, Rev C Click to download
2671 Quick Guide, Aurum Vacuum Manifold Column Purification, Rev A Click to download
2663 Quick Guide, Aurum Plasmid Mini Kit Spin Format Protocol Overview, Rev A Click to download [ Add to Cart (Free) ]
4110111 Instruction Manual, Aurum Plasmid Mini Kit, Biotechnology Explorer, Rev C Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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