Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.
|Type of support||UNOsphere S||UNOsphere Q|
|Binding Capacity||12 mg protein/column*||36 mg protein/column*|
|Column bed volume||0.2 ml||0.2 ml|
|Binding buffer||20 mM sodium acetate, pH 5.0||20 mM Tris, pH 8.3|
|Elution buffer||Binding buffer + 1.0 M NaCl||Binding buffer + 1.0 M NaCl|
|* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.|
Aurum ion exchange mini kits:
- Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
- Accommodate up to 1 ml of sample
- Use a quick, easy, spin-column format
- Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)
*U.S. patent 6,423,666.