Aurum™ Ion Exchange Mini Kits

Aurum™ Ion Exchange Mini Kits

Overview

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes.
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Description

Description

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.

  Aurum CEX Aurum AEX
Type of support UNOsphere S UNOsphere Q
Binding Capacity 12 mg protein/column* 36 mg protein/column*
Column bed volume 0.2 ml 0.2 ml
Binding buffer 20 mM sodium acetate, pH 5.0 20 mM Tris, pH 8.3
Elution buffer Binding buffer + 1.0 M NaCl Binding buffer + 1.0 M NaCl
* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.

Aurum ion exchange mini kits:

  • Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
  • Accommodate up to 1 ml of sample
  • Use a quick, easy, spin-column format
  • Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)

*U.S. patent 6,423,666.

Ordering

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7326711
Protein fractionation kit, includes cation exchange columns and buffers for basic protein enrichment, for 2 preps
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7326710
Protein fractionation kit, includes anion exchange columns and buffers for acidic protein enrichment, for 2 preps

Documents

Documents

Number Description Options
5398
Enriching Basic and Acidic Rodent Brain Proteins With Ion Exchange Spin Columns for Two-Dimensional Gel Electrophoresis, Rev A
2958
Aurum Ion Exchange Mini Kits and Columns Protocol Overview, Rev A
4110137
Instruction Manual, Aurum™ Ion Exchange Mini Kits and Columns, Rev B
2928
Aurum Ion Exchange Mini Kits and Columns Product Information Sheet, Rev A
3096
Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B