Aquadien™ Bacterial DNA Extraction and Purification Kit

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Aquadien Kit


Intended Use

The Aquadien Bacterial DNA Extraction and Purification Kit is used for reliable high-yield bacterial DNA extraction and purification from water samples to enhance sensivity and reproducibility of real-time PCR analysis. The kit must be used prior to real-time PCR assays for optimal results.

Key Benefits

  • Developed for efficient bacterial DNA extraction from all water samples, aerosols, and biofilms
  • Allows enhanced detection of bacteria in water samples, including Pseudomonas aeruginosa, Salmonella spp., and E. coli
  • Designed to work with iQ-Check® Legionella Real-Time PCR Kits or other real-time PCR analysis
  • Optimized extraction from 1 L of water per sample
  • Rapid detection and quantitation of pathogenic bacteria (24 hr)
  • Standardized protocol for routine monitoring of drinking water, swimming pools, whirlpools, industrial water distribution systems, and other water sources
  • AFNOR/ISO validated by AFNOR Certification according to the NF T90-471 and ISO TS 12869 standards

Principle

The protocol uses alkaline thermal lysis after tangential microfiltration (cross flow filtration), which reduces clogging of membrane pores during sample concentration.

Additional Information

Use the Aquadien W2 Wash Solution (3578119) for samples that clog the membrane and for samples containing PCR inhibitors.

Ordering Information

The Aquadien Bacterial DNA Extraction and Purification Kit (3578121) contains the components for processing 96 samples.

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Aquadien™ Bacterial DNA Extraction and Purification Kit

3578121
Pkg of 1 kit, includes lysis reagent, tubes, columns, and elution buffer, for 96 samples

3578121 /templatedata/internet/product_SKU/data/FSD/Water-Testing/Legionella-Testing/357-8121_1284383958_fr.xml 3578121 /default/main/bio-rad/WORKAREA/default 09/13/10 06:16 AM en_US jddgmn9w 2018-01-31 23:50:22 true true internet/product_SKU jddgmn9w templatedata/internet/product_SKU/data/FSD/Water-Testing/Legionella-Testing/357-8121_1284383958.xml FSD 2018-02-14 19:20:30 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/13/19 03:18 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-14 19:20:30 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/FSD/Water-Testing/Legionella-Testing/357-8121_1284383958.xml templatedata/internet/product_SKU/data/FSD/Water-Testing/Legionella-Testing/357-8121_1284383958.xml 3578121-aquadien-bacterial-dna-extraction-purification-kit 3578121 Aquadien Kit Aquadien&trade; Bacterial DNA Extraction and Purification Kit Pkg of 1 kit, includes lysis reagent, tubes, columns, and elution buffer, for 96 samples 96 preps, DNA extraction from water samples, includes tubes, lysis reagent, columns, and elution buffer /webroot/web/images/fsd/products/water_testing/sku_thumb/global/357-8121-aquadien-thumb.jpg /webroot/web/images/fsd/products/water_testing/sku_view/global/357-8121-aquadien-view.jpg WL Aquadien DNA Extraction Kit 96 preps, DNA extraction from water samples, includes tubes, lysis reagent, columns, and elution buffer 3578121, aquadien, dna, extraction, water, samples, testing, legionella, pneumophila, bacteria, purification, extraction, pathogen, pseudomonas aeruginosa, e. coli, e.coli, ecoli, e coli 09/13/10 06:16 AM 09/13/19 03:18 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Water-Testing/Legionella-Testing N 0 <p>Use the Aquadien Bacterial DNA Extraction and Purification Kit for the reproducible preparation of bacterial DNA from all types of water samples ready for use in real-time PCR. Detect and enumerate pathogenic bacteria including <em>Legionella</em> spp. including <em>Legionella pneumophila</em>, <em>Pseudomonas aeruginosa</em>, <em>Salmonella</em> spp., and <em>E. coli</em>.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Optimized for efficient bacterial DNA extraction from all water samples, including intra-amoeba bacteria, aerosols and biofilms</li> <li>Designed to work with <a href="/en-us/product/iq-check-legionella-real-time-pcr-kits">iQ-Check<sup>&reg;</sup> <em>Legionella</em> Real-Time PCR Kits</a> and other real-time PCR analysis</li> <li>Permits extraction from up to 1 L water per sample</li> <li>Detection and quantitation of pathogenic bacteria in 24 hr</li> <li>Use for regular monitoring of drinking water, swimming pools, whirlpools, industrial water distribution systems, and other water sources</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>R1 lysis solution &mdash; 200 ml (2 x 100)</li> <li>R2 elution buffer &mdash; 25 ml</li> <li>4.5 ml cryotubes &mdash; 100 (2 x 50)</li> <li>Purification columns &mdash; 96</li> <li>Collector vials &mdash; 192</li> </ul> <h2 class="descHeader">Validations</h2> <p>Aquadien Kits are AFNOR/ISO validated by AFNOR Certification according to the NF T90-471 and ISO TS 12869 standards</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Aquadien W2 Wash Solution (<a href="/en-us/sku/3578119-aquadien-w2-wash-solution">3578119</a>) for clogging samples and samples containing PCR inhibitors</li> <li><a href="/en-us/product/iq-check-legionella-real-time-pcr-kits">iQ-Check <em>Legionella</em> Real-Time PCR Kits</a></li> <li>Aquadien Lysis Solution (<a href="/en-us/sku/3578125-aquadien-lysis-solution">3578125</a>) for onsite collection of aerosols</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/water-pcr-testing-kits">Water PCR Testing Kits</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables?ID=027f88f2-615e-424a-803a-9baa28b55cab">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Aquadien <a href="/webroot/web/pdf/fsd/literature/881116.pdf" target="_blank">User Guide</a></li> <li>Detection of <em>Legionella</em> in Aerosols for Infection Risk Assessment <a href="/webroot/web/pdf/fsd/literature/FSD_16186.pdf" target="_blank">Tech Note</a></li> <li><em>Legionella</em> Testing Solutions <a href="/webroot/web/pdf/fsd/literature/Bulletin_16414.pdf" target="_blank">Brochure</a></li> <li>Water Safety Booklet &mdash; <a href="/webroot/web/pdf/fsd/literature/FSD_17932.pdf" target="_blank">Guide for Products in Water Testing</a></li> </ul> /webroot/web/html/fsd/products/water_testing/sku_view/description/global 357-8121-desc.html /webroot/web/html/fsd/products/water_testing/sku_view/description/global/357-8121-desc.html &nbsp; false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578121&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578121&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578121&quantity= 3578121
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Aquadien™ W2 Wash Solution

3578119
Pkg of 1, 5 ml bottle of Wash Solution W2, for use with Aquadien Bacterial DNA Extraction and Purification Kit, enough for 48 tests

2 3578119 /templatedata/internet/product_SKU/data/FSD/Water-Testing/Real-Time-PCR-Water-Testing/357-8119_en_1320570905_645.xml 3578119 /default/main/bio-rad/WORKAREA/default 07/29/11 5:00 PM en_US jbza3jt0 2017-11-14 07:07:14 true true internet/product_SKU jbza3jt0 templatedata/internet/product_SKU/data/FSD/Water-Testing/Real-Time-PCR-Water-Testing/357-8119_en_1320570905_645.xml FSD 2018-02-14 19:20:31 /default/main/bio-rad/STAGING N /default/main/bio-rad en 07/29/21 5:00 PM 1515032294952 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-14 19:20:31 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/FSD/Water-Testing/Real-Time-PCR-Water-Testing/357-8119_en_1320570905_645.xml templatedata/internet/product_SKU/data/FSD/Water-Testing/Real-Time-PCR-Water-Testing/357-8119_en_1320570905_645.xml 3578119-aquadien-w2-wash-solution 3578119 Aquadien W2, Wash Solution Aquadien™ W2 Wash Solution Pkg of 1, 5 ml bottle of Wash Solution W2, for use with Aquadien Bacterial DNA Extraction and Purification Kit, enough for 48 tests Pkg of 1, 5 ml bottle of Wash Solution W2, for use with Aquadien Bacterial DNA Extraction and Purification Kit, enough for 48 tests preview Alex Samodurov Aquadien W2, Wash Solution 5 ml, wash solution W2, for use with Aquadien DNA Extraction Kit, enough for 48 tests aquadien, dna, extraction, w2, wash, solution, clogging, clog, clogged, water, testing, legionella, 3578121, 3578119 07/29/11 5:00 PM 07/29/21 5:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Water-Testing/Real-Time-PCR-Water-Testing/ N 0 <p>Use the Aquadien W2 Wash Solution with the Aquadien Bacterial DNA Extraction and Purification Kit for samples that clog the membrane and for samples containing PCR inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Aquadien Bacterial DNA Extraction and Purification Kit (<a href="/en-us/sku/3578121-aquadien-bacterial-dna-extraction-purification-kit?ID=3578121">3578121</a>)</li> <li><a href="/en-us/product/iq-check-legionella-real-time-pcr-kits">iQ-Check<sup>&reg;</sup> <em>Legionella</em> Real-Time PCR Kits</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/water-pcr-testing-kits">Water PCR Testing Kits</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables?ID=027f88f2-615e-424a-803a-9baa28b55cab">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Aquadien <a href="/webroot/web/pdf/fsd/literature/881116.pdf" target="_blank">User Guide</a></li> <li>Detection of <em>Legionella</em> in Aerosols for Infection Risk Assessment <a href="/webroot/web/pdf/fsd/literature/FSD_16186.pdf" target="_blank">Tech Note</a></li> <li><em>Legionella</em> Testing Solutions <a href="/webroot/web/pdf/fsd/literature/Bulletin_16414.pdf" target="_blank">Brochure</a></li> <li>Water Safety Booklet &mdash; <a href="/webroot/web/pdf/fsd/literature/FSD_17932.pdf" target="_blank">Guide for Products in Water Testing</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated 3578119.html /webroot/web/html/fsd/products/SKUs/autopopulated/3578119.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578119&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578119&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578119&quantity= 3578119
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Aquadien™ Lysis Solution

3578125
Pkg of 1, 100 ml bottle, for use with the CIP10-M Air Sampler, sufficient for 32 air samples

3578125 /templatedata/internet/product_SKU/data/FSD/Water-Testing/Pathogenic-Germs/357-8125_en_1320570905_647.xml 3578125 /default/main/bio-rad/WORKAREA/default 07/29/11 5:00 PM en_US jbza3jt5 2017-11-14 07:07:13 true true internet/product_SKU jbza3jt5 templatedata/internet/product_SKU/data/FSD/Water-Testing/Pathogenic-Germs/357-8125_en_1320570905_647.xml FSD 2018-02-14 19:20:31 /default/main/bio-rad/STAGING N /default/main/bio-rad en 07/29/21 5:00 PM 1515032294910 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-14 19:20:31 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/FSD/Water-Testing/Pathogenic-Germs/357-8125_en_1320570905_647.xml templatedata/internet/product_SKU/data/FSD/Water-Testing/Pathogenic-Germs/357-8125_en_1320570905_647.xml 3578125-aquadien-lysis-solution 3578125 Aquadien Lysis Solution; sufficient for 100 samples Aquadien&trade; Lysis Solution Pkg of 1, 100 ml bottle, for use with the CIP10-M Air Sampler, sufficient for 32 air samples 100 ml x 1 bottle, sufficient for 32 air samples /webroot/web/images/fsd/products/water_testing/sku_thumb/global/aquadien-lysis-solution-3578125-thumb.jpg /webroot/web/images/fsd/products/water_testing/sku_view/global/aquadien-lysis-solution-3578125-view.jpg preview Alex Samodurov Aquadien Lysis Solution; sufficient for 100 samples 100 ml x 1 bottle 3578125, aquadien, cip10-m, lysis solution, microorganisms, micro-organisms, collect, collection 07/29/11 5:00 PM 07/29/21 5:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Water-Testing/Pathogenic-Germs/ N 0 <p>Use the Aquadien Lysis Solution in the compact, portable CIP10-M Air Sampler to collect microorganisms from local aerosols. DNA collected can be concentrated and purified with the <a href="/en-us/sku/3578121-aquadien-bacterial-dna-extraction-purification-kit?ID=3578121">Aquadien Bacterial DNA Extraction and Purification Kit</a>. Samples can be analyzed using <a href="/en-us/product/iq-check-legionella-real-time-pcr-kits">iQ-Check<sup>&reg;</sup> <em>Legionella</em> Real-Time PCR Kits</a> or other real-time PCR analyses.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li><a href="/en-us/product/cip10-m-air-sampler?pcp_loc=catprod">CIP10-M Air Sampler</a> with Aquadien Lysis Solution is compact and portable</li> <li>Evaluate aerosols around any water source</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>Aquadien Bacterial DNA Extraction and Purification Kit (<a href="/en-us/sku/3578121-aquadien-bacterial-dna-extraction-purification-kit?ID=3578121">3578121</a>)</li> <li>Aquadien W2 Wash Solution (<a href="/en-us/sku/3578119-aquadien-w2-wash-solution">3578119</a>) for clogging samples and samples containing PCR inhibitors</li> <li><a href="/en-us/product/iq-check-legionella-real-time-pcr-kits">iQ-Check <em>Legionella</em> Real-Time PCR Kits</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-fr/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-fr/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Aquadien <a href="/webroot/web/pdf/fsd/literature/881116.pdf" target="_blank">User Guide</a></li> <li>Detection of <em>Legionella</em> in Aerosols for Infection Risk Assessment <a href="/webroot/web/pdf/fsd/literature/FSD_16186.pdf" target="_blank">Tech Note</a></li> <li><em>Legionella</em> Testing Solutions <a href="/webroot/web/pdf/fsd/literature/Bulletin_16414.pdf" target="_blank">Brochure</a></li> <li>Water Safety Booklet &mdash; <a href="/webroot/web/pdf/fsd/literature/FSD_17932.pdf" target="_blank">Guide for Products in Water Testing</a></li> </ul> /webroot/web/html/fsd/products/water_testing/sku_view/description/global aquadien-lysis-solution-3578125-desc.html /webroot/web/html/fsd/products/water_testing/sku_view/description/global/aquadien-lysis-solution-3578125-desc.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578125&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578125&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578125&quantity= 3578125
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Number Description Options
881116 Aquadien™ Kit User Guide Click to download
16414 Legionella Testing Solutions Brochure, Ver E Click to download
FSD_17932 Water Safety Booklet - Guide for Products in Water Testing Click to download

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