Anti-Cardiolipin (aCL) Assays

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Anti-Cardiolipin (aCL) Assays

Overview

Anti-Cardiolipin Assays

Anti-Cardiolipin Autoimmune EIA Overview

Anti-Cardiolipin (aCL) Testing

The aCL assay serves to assist in the diagnosis of antiphospholipid syndrome. This diagnosis has important implications for the treatment and prediction of recurrent thrombosis and recurrent miscarriage.

The Bio-Rad Anti-Cardiolipin Test Kits are enzyme-linked immunosorbent assays (ELISA) for the detection and semi-quantitative determination of aCL IgG, IgM, or IgA antibodies in human serum or plasma. The results are reported in GPL, MPL, and APL units that are directly traceable to an internationally recognized reference preparation. The Bio-Rad Anti-Cardiolipin Test Kits are intended to be used by clinical (hospital and reference) laboratories.

Product Highlights

  • Specific determinations of IgG, IgM, and IgA aCL antibodies
  • Flexible multi-point or single-point calibration options
  • Better regression analysis with distinct calibrators
  • Built-in quality control with undiluted controls and calibrators

 

Anti-Cardiolipin Assays

Anti-Cardiolipin Autoimmune EIA

Pre-Test Setup Instructions


Step 1. Bring all reagents to room temperature (18–26°C).


Step 2. Prepare the Wash Solution.


Step 3. Make 1:50 working solutions by diluting 10 µL of patient's serum positive/negative controls, and 3 calibrators in 490 µL of diluent.


Test Procedure


Step 1. Apply 100 µL diluted patient samples, controls, and calibrator to wells.

Step 2. Incubate wells for 30–40 minutes at room temperature (18–26°C).


Step 3. Wash wells 4x with ~250 µL of wash solution.Remove all liquid before proceeding.


Step 4. Apply 100 µL HRP conjugate to all wells. Discard excess transferred HRP after use.


Step 5. Incubate wells for 30–40 minutes at room temperature (18–26°C).


Step 6. Wash wells 4x with ~250 µL of wash solution. Remove all liquid before proceeding.


Step 7. Develop color by adding 100 µL substrate to each well. Discard excess transferred substrate after use.


Step 8. Incubate for 30–40 minutes at room temperature (18–26°C)


Step 9. Stop color development by adding 100 µL of stop solution to each well.


Step 10. Read results within 30 minutes with an EIA reader set to 450 nm.

Anti-Cardiolipin Assays

Anti-Cardiolipin Autoimmune EIA

aPL Testing Algorithm: Antiphospholipid Antibody Screening Procedure

Screen with aCL and/or aPS (IgG, IgM, IgA)*

Test for anti-β2 GPI (IgG, IgM, IgA)*

Repeat testing in at least six weeks for seroconversion or antibody persistance


* Screen for each isotype. Polyvalent screen not recommended.

APS = Antiphospholipid syndrome
aCL = Anti-cardiolipin
aPS = Anti-phosphatidylserine
β2 GPI = β-2 glycoprotein 1 (cofactor)


Cut-Off Value

  • aCL IgG: <23 GPL units
  • aCL IgA: <22 APL units
  • aCL IgM: <11 MPL units
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Anti-Cardiolipin (aCL) Assays are intended for the semi-quantitative determination of aCL antibodies in human serum or plasma. Anti-cardiolipin antibodies are frequently found in patients with systemic lupus erythematosus (SLE). Elevated levels of aCL antibodies have been significantly associated with the presence of both venous and arterial thrombosis, thrombocytopenia, and recurrent pregnancy loss.

All Bio-Rad Anti-Cardiolipin (aCL) Assays feature common protocols and common reagents. Reagents and reagent bottle caps are color-coded for ease of use. Test operation is further streamlined with breakaway wells, easy automation, a linear calibration curve and flexible single-point and multi-point calibration options. Quality controls are built in, with undiluted test controls and calibrators.

Assay Characteristics

  • Sample dilution: 1:50
  • Incubation time: 30/30/30 minutes
  • Incubation temperature: room temperature (18–26°C)
  • Conjugate: HRP
  • Substrate: TMB


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Anti-Cardiolipin IgG EIA Kit

425-2000
Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgG antibodies (96 tests)

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Anti-Cardiolipin IgM EIA Kit

425-2020
Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgM antibodies (96 tests)

1 4252020 /templatedata/internet/product_SKU/data/CDG/Autoimmune/Anti-Phospholipid-Testing/425-2020_0921120816787.xml 4252020 09/21/09 12:08 PM N en 09/21/19 12:08 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:18 4252020-anti-cardiolipin-igm-eia-kit 4252020 Anti-Cardiolipin IgM EIA Kit Anti-Cardiolipin IgM EIA Kit Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgM antibodies (96 tests) Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgM antibodies (96 tests) Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgM antibodies (96 tests) /webroot/web/images/cdg/products/autoimmune/sku_thumb/global/aibu_425_2020_tn.jpg /webroot/web/images/cdg/products/autoimmune/sku_view/global/aibu_425_2020_view.jpg /webroot/web/images/cdg/products/autoimmune/sku_feature/global/aibu_425_2020_feature.jpg RepEx 425-2020 Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgM antibodies (96 tests) 09/21/09 12:08 PM 09/21/19 12:08 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Autoimmune/Anti-Phospholipid-Testing N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252020&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252020&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252020&quantity= 425-2020
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Anti-Cardiolipin IgA EIA Kit

425-2040
Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgA antibodies (96 tests)

2 4252040 /templatedata/internet/product_SKU/data/CDG/Autoimmune/Anti-Phospholipid-Testing/425-2040_0921120816768.xml 4252040 09/21/09 12:08 PM N en 09/21/19 12:08 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:18 4252040-anti-cardiolipin-iga-eia-kit 4252040 Anti-Cardiolipin IgA EIA Kit Anti-Cardiolipin IgA EIA Kit Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgA antibodies (96 tests) Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgA antibodies (96 tests) Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgA antibodies (96 tests) /webroot/web/images/cdg/products/autoimmune/sku_thumb/global/aibu_425_2040_tn.jpg /webroot/web/images/cdg/products/autoimmune/sku_view/global/aibu_425_2040_view.jpg /webroot/web/images/cdg/products/autoimmune/sku_feature/global/aibu_425_2040_feature.jpg RepEx 425-2040 Semi-quantitative enzyme immunoassay for detection of anti-cardiolipin IgA antibodies (96 tests) 09/21/09 12:08 PM 09/21/19 12:08 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Autoimmune/Anti-Phospholipid-Testing N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252040&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252040&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4252040&quantity= 425-2040
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Q-730 Anti-Cardiolipin Product Sheet Click to download [ Add to Cart (Free) ]

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