Analytical Grade Cation Exchange Media

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Analytical Grade Cation Exchange Media - Cation Exchange Resin

Overview

Specifications
AG® 50W Resins
Resin Crosslinkage Approx.
MW Exclusion
Operating Stability/pH Range Min. Wet Capacity (meq/ml) Nominal Density (g/ml) Ionic Forms
Type: Strong cation 2% 2,700 0–14/0–14 0.6 0.7 Hydrogen
Functional group: R—SO3 - 4% 1,400   1.1 0.8 Hydrogen
Matrix: Styrene divinylbenzene 8% 1,000   1.7 0.8 Hydrogen
  12% 400   2.1 0.085 Hydrogen

Applications: Lower crosslinkage resins are used for separation or concentration of peptides, nucleotides, and amino acids. Higher crosslinkage resins are useful for separations of smaller peptides and amino acids, removal of cation, and separation of metal ions.

AG MP-50 Resin
Resin Crosslinkage Approx.
MW Exclusion
Operating Stability/pH Range Min. Wet Capacity (meq/ml) Nominal Density (g/ml) Ionic Forms
Type: Strong cation Macroporous >1,000,000 0–14/0–14 1.5 0.8 Hydrogen
Functional Group: R-SO3-  
Matrix: Styrene divinylbenzene  

Applications: Macroporous equivalent of AG 50W resin. Its effective surface area is approximately 35 m2 per dry gram, which is equivalent to 30-35% porosity. It can be used for adsorption of Ca2+, Mg2+, Na2+, and antibiotics.

Bio-Rex 70 Resin
Resin Crosslinkage Approx.
MW Exclusion
Operating Stability/pH Range Min. Wet Capacity (meq/ml) Nominal Density (g/ml) Ionic Forms
Type: Weak cation Macroreticular >1,000,000 5–14/0–14 2.4 0.7 Sodium
Functional Group: R–COO–  
Matrix: Acrylic  

Applications: High capacity for high molecular weight solutes; does not denature proteins. Used for purification and fractionation of peptidesand proteins.

Analytical Grade Resin Wet Mesh and Equivalent Diameters

Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38
Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
Various Counterions
Counterion Relative Selectivity for AG 50W-X8 Resin Counterion Relative Selectivity for AG 50W-X8 Resin
 
H+ 1.0 Fe2+ 2.55
Li+ 0.85 Zn2+ 2.7
Na+ 1.5 Co2+ 2.8
NH4+ 1.95 Cu2+ 2.9
K+ 2.5 Cd2+ 2.95
Rb+ 2.6 Ni2+ 3.0
Cs+ 2.7 Ca2+ 3.9
Cu+ 5.3 Sr3+ 4.95
Ag+ 7.6 Hg3+ 7.2
Mn2+ 2.35 Ph2+ 7.5
Mg3+ 2.5 Ba3+ 8.7
 
Resin Conversion
  AG 50® resin Bio-Rex®
MSZ 50 resin
Conversion from/to H+ Na+ H+ pyridinium
Reagent used 1 M NaOH 1M pyridine (wash with H2O before pyridine)
Volumes of Sol'n/vol. of resin 2 2
Flow rate ml/min/cm2 of bed 2 1
Type of exchange N N
Test for completeness of conversion pH 9
Rinse: vol. DI water/vol.resin 4
Test for completion of rinsing pH <9

AG®, AG-MP, Bio-Rex®, and Chelex® Resins

AG (Analytical grade) resins are extensively purified to remove both organic and inorganic impurities. Resins are sized to consistently give narrow wet mesh ranges, which provide high resolution and excellent reproducibility. AG resins are available as both strong and weak cation and anion exchangers and as mixed-bed ion exchangers. Many are available in several ionic forms and can be converted from one form to another. AG resins are primarily used for the separation of low molecular weight compounds such as inorganic ions, organic acids, nucleic acids, or carbohydrates.

AG 50W resin, AG MP-50 macroporous resin, and biotechnology grade AG 50W all offer strong acid cation exchange capabilities. The AG resin has been exhaustively sized, purified, and converted to provide accurate, reproducible analytical results. Both resins are useful for single step purification methods, for concentrating cationic solutes, and for analytical determination of various mixed cationic solutes. Both AG 50W and MP-50 have sulfonic acid functional groups attached to a styrene divinylbenzene copolymer lattice. The amount of resin crosslinking determines the bead pore size: a resin with a lower crosslinkage has a more open structure that is more permeable to high molecular weight substances than a highly crosslinked resin.

AG MP-50 resin is the macroporous equivalent of AG 50W resin. Its effective surface area approximates 35 square meters per dry gram, or 30–35% porosity.

Bio-Rex 70 resin has a carboxylic acid functional group attached to an acrylic matrix. It is a macroreticular resin with a high capacity for high molecular weight compounds and does not denature proteins. It is used for purification and fraction of peptides and proteins.

Chelex 100 resin contains paired iminodiacetate ions coupled to a styrene divinylbenzene support. They are unique chelating resins that bind polyvalent cations with high selectivity and are used to remove metal ions from samples and buffers.

Molecular Biology and Biotechnology grade resins

Molecular biology grade resins, chemically identical to the equivalent analytical grade resins, are certified to be free of endo- and exonuclease activities and ligase inhibitors.

Biotechnology grade resins have undergone special processing and contain fewer than 100 microorganisms/g.

Larger volumes and special packaging for industrial applications are available on request.

 

AG 50W-X2 Resin #142-1231
AG 50W-X2 Resin

142-1231
500 g, analytical grade cation exchange resin, hydrogen form, 2% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~2,700 MW limit

List Price:   $989.00
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AG 50W-X2 Resin #142-1241
AG 50W-X2 Resin

142-1241
500 g, analytical grade cation exchange resin, hydrogen form, 2% crosslinkage, 100–200 dry mesh size, 106–300 µm wet bead size, ~2,700 MW limit

List Price:   $681.00
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AG 50W-X2 Resin #142-1251
AG 50W-X2 Resin

142-1251
500 g, analytical grade cation exchange resin, hydrogen form, 2% crosslinkage, 200–400 dry mesh size, 75–180 µm wet bead size, ~2,700 MW limit

List Price:   $1,214.00
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AG 50W-X4 Resin #142-1331
AG 50W-X4 Resin

142-1331
500 g, analytical grade cation exchange resin, hydrogen form, 4% crosslinkage, 50–100 dry mesh size, 180–425 µm wet bead size, ~1,400 MW limit

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AG 50W-X4 Resin #142-1341
AG 50W-X4 Resin

142-1341
500 g, analytical grade cation exchange resin, hydrogen form, 4% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~1,400 MW limit

List Price:   $553.00
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AG 50W-X4 Resin #142-1351
AG 50W-X4 Resin

142-1351
500 g, analytical grade cation exchange resin, hydrogen form, 4% crosslinkage, 200–400 dry mesh size, 63–150 µm wet bead size, ~1,400 MW limit

List Price:   $522.00
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AG 50W-X8 Resin #142-1421
AG 50W-X8 Resin

142-1421
500 g, analytical grade cation exchange resin, hydrogen form, 8% crosslinkage, 20–50 dry mesh size, 300–1,180 µm wet bead size, ~1,000 MW limit

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AG 50W-X8 Resin #142-1431
AG 50W-X8 Resin

142-1431
500 g, analytical grade cation exchange resin, hydrogen form, 8% crosslinkage, 50–100 dry mesh size, 180–425 µm wet bead size, ~1,000 MW limit

List Price:   $681.00
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AG 50W-X8 Resin #142-1441
AG 50W-X8 Resin

142-1441
500 g, analytical grade cation exchange resin, hydrogen form, 8% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~1,000 MW limit

List Price:   $579.00
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AG 50W-X8 Resin #142-1451
AG 50W-X8 Resin

142-1451
500 g, analytical grade cation exchange resin, hydrogen form, 8% crosslinkage, 200–400 dry mesh size, 63–150 µm wet bead size, ~1,000 MW limit

List Price:   $686.00
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AG 50W-X12 Resin #142-1641
AG 50W-X12 Resin

142-1641
500 g, analytical grade cation exchange resin, hydrogen form, 12% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~400 MW limit

List Price:   $1,219.00
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AG 50W-X12 Resin #142-1651
AG 50W-X12 Resin

142-1651
500 g, analytical grade cation exchange resin, hydrogen form, 12% crosslinkage, 200–400 dry mesh size, 53–106 µm wet bead size, ~400 MW limit

List Price:   $1,942.00
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Bio-Rex 70 Resin

142-5822
500 g, cation exchange resin, sodium form, macroreticular, 20–50 dry mesh size, 300–1,180 µm wet bead size, >1,000,000 MW limit

List Price:   $748.00
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Bio-Rex 70 Resin

142-5832
500 g, cation exchange resin, sodium form, macroreticular, 50–100 dry mesh size, 150–300 µm wet bead size, >1,000,000 MW limit

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Bio-Rex 70 Resin

142-5842
500 g, cation exchange resin, sodium form, macroreticular, 100–200 dry mesh size, 75–150 µm wet bead size, >1,000,000 MW limit

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Bio-Rex 70 Resin

142-5852
500 g, cation exchange resin, sodium form, macroreticular, 200–400 dry mesh size, 45–75 µm wet bead size, >1,000,000 MW limit

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AG 50W-X8 Resin #143-1451
AG 50W-X8 Resin

143-1451
100 g, molecular biology grade cation exchange resin, sodium form, 8% crosslinkage, 200–400 dry mesh size, 63–150 µm wet bead size, ~1,000 MW limit

List Price:   $616.00
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Chelex 100 Resin

142-1253
50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit

List Price:   $184.00
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AG 50W-X2 Resin #143-5241
AG 50W-X2 Resin

143-5241
100 g, biotechnology grade cation exchange resin, hydrogen form, 2% crosslinkage, 100–200 dry mesh size, 106–300 µm wet bead size, ~2,700 MW limit

List Price:   $374.00
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AG 50W-X4 Resin #143-5341
AG 50W-X4 Resin

143-5341
100 g, biotechnology grade cation exchange resin, hydrogen form, 4% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~1,400 MW limit

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AG 50W-X8 Resin #143-5441
AG 50W-X8 Resin

143-5441
100 g, biotechnology grade cation exchange resin, hydrogen form, 8% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~1,000 MW limit

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AG 50W-X8 Resin #143-5451
AG 50W-X8 Resin

143-5451
100 g, biotechnology grade cation exchange resin, hydrogen form, 8% crosslinkage, 200–400 dry mesh size, 63–150 µm wet bead size, ~1,000 MW limit

List Price:   $374.00
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Chelex 100 Resin

142-2832
500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

List Price:   $743.00
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Bio-Rex 70 Resin

143-5832
100 g, biotechnology grade cation exchange resin, sodium form, macroreticular, 50–100 dry mesh size, 150–300 µm wet bead size, >1,000,000 MW limit

List Price:   $374.00
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Bio-Rex 70 Resin

143-5852
100 g, biotechnology grade cation exchange resin, sodium form, macroreticular, 200–400 dry mesh size, 45–75 µm wet bead size, >1,000,000 MW limit

List Price:   $374.00
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Number Description Options
1801 Protein and Peptide Purification Applications, Rev B Click to download
LIT207 Instruction Manual, AG 3, AG 4, and Bio-Rex Anion Exchange Resins Click to download [ Add to Cart (Free) ]
LIT206 Instruction Manual, Bio-Rex Weakly Acidic Cation Exchange Resin, Rev B Click to download
LIT304 Instruction Manual, Molecular Biology Grade AG 50W-X8 Cation Exchange Resin, Rev B Click to download [ Add to Cart (Free) ]
LIT203 Instruction Manual, AG 50W and AG MP-50 Cation Exchange Resins, Rev B Click to download