AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

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AL Agar Listeria according to Ottaviani and Agosti Medium

Overview

Key Benefits
  • Economical: 1 broth + 1 plate
  • Fast: 24-hour results after enrichment
  • Easy to read: blue colony with/without halo
  • Flexible: detection, enumeration or ISO 11290-1 and -2 standard
  • Reliable: NF validation (ISO 16140)


Protocol
Detection of Campylobacter spp.
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/16-01/09
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The AL protocol is certified by AFNOR Certification, according to the ISO 16140 requirements as an alternative method to the NF EN ISO 11290-1 reference standard, for the Listeria monocytogenes and other Listeria species under BRD 07/16-01/09.

Nordval Validation Ref No032.



Intended Use

AL Medium (Agar Listeria according to Ottaviani and Agosti) is based on the simultaneous detection of two enzyme activities: β-glucosidase and phosphatidylinositol-specific phospholipase C (PI-PLC).

Key Benefits

  • Detection of Listeria spp. in 24 hr after 24 hr of enrichment
  • Only1 plate needed per sample
  • Easy-to-read chromogenic reaction: blue colonies are Listeria spp., very easy to read on the whitish plate
  • Highly specific: tested on 50 strains of Listeria spp. and 30 strains of non-Listeria species
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

β-D-glucosidase activity, common to the Listeria genus, is detected using a chromogenic substrate (X-glucoside). Its hydrolysis induces the formation of a blue to blue-green color in all Listeria colonies.

PI-PLC is an enzyme only detected in pathogenic Listeria species: L. monocytogenes and L. ivanovii. AL medium contains phosphatidylinositol which, when it breaks down, produces an opaque halo around the colonies of these two bacterial species. The halo is visible after 24 hr for L. monocytogenes and 48 hr for L. ivanovii.

AL (Agar Listeria according to Ottaviani and Agosti) Chromogenic Assay

Enzymatic Activity L. monocytogenes Listeria spp. Non-Listeria
Pl-PLC dectection + - -
β-D-Glucosidase + + -
     

Ordering Information

AL (Agar Listeria according to Ottaviani and Agosti) is offered as ready-to-use plates (3563695), agar (3555200), or dehydrated agar broth (3564043).

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AL (Agar Listeria according to Ottaviani and Agosti) Agar Plates

3563695
Pkg of 20, 90 mm agar plates

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$80.00
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AL (Agar Listeria according to Ottaviani and Agosti) Agar Base, dehydrated

3564043
Pkg of 1, 500 g of dehydrated broth, enough for 7.2 L of medium

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$501.00
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AL (Agar Listeria according to Ottaviani and Agosti) Agar with Supplement 2

3564042
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium

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$307.00
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Fraser 1/2 Broth #3555797
Fraser 1/2 Broth, ready to use

3555797
Pkg of 6, 225 ml bottles

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$41.00
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Fraser Broth Base #356-4604
Fraser Broth Base, dehydrated

3564604
Pkg of 1, 500 g of dehydrated broth base

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$115.00
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Fraser 1/2 Supplement #356-4616
Fraser 1/2 Strength Supplement

3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium

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$200.00
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Buffered Peptone Water Broth, ready to use

3554179
Pkg of 6, 225 ml bottles

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$27.00
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(Discontinued) Buffered Peptone Water Broth, ready to use

3555790
Pkg of 2, 5 L bags

This product is no longer available. Please contact technical support for more information on replacement product.
Buffered Peptone Water Broth, dehydrated

3564684
Pkg of 1, 500 g

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$58.00
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iQ-Check Listeria spp. kit #357-8113
iQ-Check® Listeria spp. PCR Detection Kit

3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check Listeria monocytogenes II kit #357-8124
iQ-Check® Listeria monocytogenes II PCR Detection Kit

3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_AL_en AL Agar (Agar Listeria according to Ottaviani and Agosti) Technical Sheet (All countries except US and Canada) Click to download
FT_AL_fr AL Gélose (Agar Listeria selon Ottaviani et Agosti) Fiche Technique (Tous pays, sauf USA and Canada) Click to download