AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

Selective chromogenic medium used for the detection and enumeration of Listeria monocytogenes in food and environmental samples.

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Overview

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AL Medium (Agar Listeria according to Ottaviani and Agosti) is based on the simultaneous detection of two enzyme activities: β-glucosidase and phosphatidylinositol-specific phospholipase C (PI-PLC).

Key Benefits

  • Detection of Listeria spp. in 24 hr after 24 hr of enrichment
  • Only1 plate needed per sample
  • Easy-to-read chromogenic reaction: blue colonies are Listeria spp., very easy to read on the whitish plate
  • Highly specific: tested on 50 strains of Listeria spp. and 30 strains of non-Listeria species
  • Certified NF VALIDATION according to the ISO 16140 standard

Principle

β-D-glucosidase activity, common to the Listeria genus, is detected using a chromogenic substrate (X-glucoside). Its hydrolysis induces the formation of a blue to blue-green color in all Listeria colonies.

PI-PLC is an enzyme only detected in pathogenic Listeria species: L. monocytogenes and L. ivanovii. AL medium contains phosphatidylinositol which, when it breaks down, produces an opaque halo around the colonies of these two bacterial species. The halo is visible after 24 hr for L. monocytogenes and 48 hr for L. ivanovii.

AL (Agar Listeria according to Ottaviani and Agosti) Chromogenic Assay

Enzymatic Activity
L. monocytogenes
Listeria spp.
Non-Listeria
Pl-PLC dectection yes no no
β-D-Glucosidase yes yes no

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Pkg of 6, 225 ml bottles
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3564604
Pkg of 1, 500 g of dehydrated broth base
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3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium
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3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Documents

Number Description Options
10000135677
AL Agar, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7408
Chromogenic Media Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000147404
Rhamnose Test, UserGuide (English, French, German, Italian, Portuguese, Spanish)