AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

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AL Agar Listeria according to Ottaviani and Agosti Medium

Overview

Key Benefits
  • Economical: 1 broth + 1 plate
  • Fast: 24-hour results after enrichment
  • Easy to read: blue colony with/without halo
  • Flexible: detection, enumeration or ISO 11290-1 and -2 standard
  • Reliable: NF validation (ISO 16140)


Protocol
Detection of Campylobacter spp.
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/16-01/09
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The AL protocol is certified by AFNOR Certification, according to the ISO 16140 requirements as an alternative method to the NF EN ISO 11290-1 reference standard, for the Listeria monocytogenes and other Listeria species under BRD 07/16-01/09.

Nordval Validation Ref No032.

AL medium (Agar Listeria according to Ottaviani and Agosti) is based on the simultaneous detection of 2 enzyme activities: b-glucosidase and phosphatidylinositol-specific phospholipase C (PI-PLC).

B-D-glucosidase activity, common to Listeria genus, is detected using a chromogenic substrate (X-glucoside). Its hydrolysis induces the formation of a blue to blue-green colour in all Listeria colonies.

PI-PLC is an enzyme only detected in pathogenic Listeria species: L. monocytogenes and L. ivanovii. AL medium contains phosphatidylinositol which, when it breaks down, produces an opaque halo around colonies of bacteria of these 2 species: 24 hrs for L. monocytogenes and 48 hrs for L. ivanovii.

Fraser 1/2 Broth #355-5797
Fraser 1/2 Broth

355-5797
225 ml x 6 bottles

List Price:   $41.00
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Fraser Broth Base #356-4604
Fraser Broth Base

356-4604
500 g

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Fraser 1/2 Supplement #356-4616
Fraser 1/2 Supplement

356-4616
10 vials (1 vial q.s.p. 2.25 l)

List Price:   $91.00
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Buffered Peptone Water/Broth

355-4179
225 ml x 6 bottles

List Price:   $25.00
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Buffered Peptone Water/Broth

355-5790
5 L x 2 bags

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Buffered Peptone Water/Broth

356-4684
500 g

List Price:   $58.00
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Number Description Options
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Brochure, Rev H Click to download
FSD_17147 AL(Agar Listeria according to Ottaviani and Agosti), Product Information, Rev D Click to download
PF_261 Gélose AL (Agar Listeria selon Ottaviani et Agosti) Information Produit (Tous pays, sauf USA) Click to download
TS_AL_en AL short protocol/Agar (Agar Listeria according to Ottaviani and Agosti) Technical Sheet (All countries except US and Canada) Click to download
FT_AL_fr AL protocole court/Gélose (Agar Listeria selon Ottaviani et Agosti) Fiche Technique (Tous pays, sauf USA and Canada) Click to download