AG® 1 and AG® 2 Anion Exchange Resins

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Overview

Specifications of AG® 1 and AG® 2 Resins
AG 1 Resins
  Crosslinkage Approx. MW Exclusion Operating Stability/pH Range Min. Wet Capacity (meq/ml) Nominal Density (g/ml) Ionic Forms
Type: Strong anion 2% 2,700 0–14/0–14 0.6 0.65 Chloride, acetate
Functional group: R-CH2N-(CH3)3 4% 1,400   1 0.7 Chloride
Matrix: Styrene divinylbenzene 8% 1,000   1.2 0.75 Chloride, hydroxide, acetate, formate

Applications: Exchange of anions of salts and ampholytes ion basic form (at pH>pl ). Lower crosslinkages are useful for higher molecular weight molecules, such as peptides, nucleotides, and uranium complexes. Higher crosslinkages are useful for low molecular weight inorganic anions and for applications such as cyclic nucleotide assays and fractionation of organic acids.


AG 2 Resins
  Crosslinkage Approx. MW Exclusion Operating Stability/pH Range Min. Wet Capacity (meq/ml) Nominal Density (g/ml) Ionic Forms
Type: Strong anion 8% 1,000 0-14/0-14 1.2 0.75 Chloride
Functional group: R-CH2N-(CH3)3  
Matrix: Styrene divinylbenzene  

Applications: Similar to AG 1 resin but less basic and less resistant to oxidation, AG 2 resin is capable of separating sugars, sugar alcohols, and gluocosides. The isomerization of some sugars, which occurs with AG 1 resin, does not occur with AG 2 resin.

Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
AG Resin Wet Mesh and Diameters
Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38
Relative Selectivity of Various Counterions on Selected AG Resins
AG 50W-X8 Resin
AG 1-X8 Resin
Counterion
Relative Selectivity
Counterion
Relative Selectivity
H+
1.0
OH–
1.0
Li+
0.85
F–
1.6
Na+
1.5
Propionate
2.6
NH4+
1.95
Acetate
3.2
Mn2+
2.35
Formate
4.6
K+
2.5
HPO4
5.0
Mg2+
2.5
IO3
5.5
Fe2+
2.55
HCO3
6.0
Rb+
2.6
Cl–
22
Cs+
2.7
NO2
24
Zn2+
2.7
BrO3
27
Co2+
2.8
HSO3
27
Cu2+
2.9
CN–
28
Cd2+
2.95
Br–
50
Ni2+
3.0
NO3
65
Ca2+
3.9
ClO3
74
Sr2+
4.95
HSO4
85
Cu+
5.3
Phenate
110
Hg2+
7.2
I–
175
Pb2+
7.5
Citrate
220
Ag+
7.6
Salicylate
450
Ba2+
8.7
Benzenesulfonate
500
Selecting Cation or Anion Exchange Support

Separation strategy for amphoteric molecules. If the molecule is stable at a pH above its pl, an anion exchange support is used. If the molecule is stable at a pH below its pl, a cation exchange support is used.

AG (analytical grade) resins are extensively purified to remove both organic and inorganic impurities. They are also sized to consistently give narrow wet mesh ranges, which provide high resolution and excellent reproducibility. AG resins are available as both strong and weak anion and cation exchangers and as mixed-bed ion exchangers. Many are available in several ionic forms and can be converted from one form to another. AG resins are used primarily for the separation of low molecular weight compounds such as inorganic ions, organic acids, nucleic acids, or carbohydrates.

AG 1 and AG 2 resins are strongly basic anion exchangers with quaternary ammonium functional groups attached to the styrene divinylbenzene copolymer lattice. The amount of resin crosslinkage determines the bead pore size. A resin with a lower percentage of crosslinkage has a more open structure permeable to higher molecular weight substances than a highly crosslinked resin. It also has a lower physical resistance to shrinking and swelling, so that it absorbs more water and swells to a larger wet diameter than a highly crosslinked resin of equivalent dry diameter.  For example, the lower crosslinked resins, particularly AG 1-X2 2% crosslinked resin, are useful for the sorption and fractionation of relatively high molecular weight substances such as peptides, ribo- and deoxyribonucleotides, and uranium. The higher crosslinked resins, particularly AG 1-X8 8% crosslinked resin, are used for sorption, exchange, and separation of low molecular weight inorganic anions and in applications such as cyclic nucleotide assays and fractionation of organic acids.

AG 2 resin is similar to AG 1 resin, but is slightly less basic and slightly less resistant to oxidation due to differences in the structure of the quaternary functional group. It offers advantages in certain applications. For example, it is capable of separating sugars, sugar alcohols, and glycosides using a step gradient and borate buffers without isomerizing some sugars.

Larger volumes and special packaging for industrial applications are available on request.

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AG 1-X2 Resin

140-1231
500 g, analytical grade anion exchange resin, chloride form, 2% crosslinkage, 50–100 dry mesh size, 180–500 µm wet bead size, ~2,700 MW limit

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AG 1-X2 Resin

140-1241
500 g, analytical grade anion exchange resin, chloride form, 2% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~2,700 MW limit

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AG 1-X2 Resin #140-1251
AG 1-X2 Resin

140-1251
500 g, analytical grade anion exchange resin, chloride form, 2% crosslinkage, 200–400 dry mesh size, 75–180 µm wet bead size, ~2,700 MW limit

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AG 1-X2 Resin #140-1253
AG 1-X2 Resin

140-1253
500 g, analytical grade anion exchange resin, acetate form, 2% crosslinkage, 200–400 dry mesh size, 75–180 µm wet bead size, ~2,700 MW limit

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AG 1-X2 Resin #140-1331
AG 1-X4 Resin

140-1331
500 g, analytical grade anion exchange resin, chloride form, 4% crosslinkage, 50–100 dry mesh size, 180–425 µm wet bead size, ~1,400 MW limit

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AG 1-X2 Resin #140-1341
AG 1-X4 Resin

140-1341
500 g, analytical grade anion exchange resin, chloride form, 4% crosslinkage, 100–200 dry mesh size, 106–250 µm wet bead size, ~1,400 MW limit

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AG 1-X2 Resin #140-1351
AG 1-X4 Resin

140-1351
500 g, analytical grade anion exchange resin, chloride form, 4% crosslinkage, 200–400 dry mesh size, 63–150 µm wet bead size, ~1,400 MW limit

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AG 1-X2 Resin #140-1421
AG 1-X8 Resin

140-1421
500 g, analytical grade anion exchange resin, chloride form, 8% crosslinkage, 20–50 dry mesh size, 300–1,180 µm wet bead size, ~1,000 MW limit

List Price:   $333.00
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AG 1-X2 Resin #140-1431
AG 1-X8 Resin

140-1431
500 g, analytical grade anion exchange resin, chloride form, 8% crosslinkage, 50–100 dry mesh size, 180–425 µm wet bead size, ~1,000 MW limit

List Price:   $512.00
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AG 1-X2 Resin #140-1441
AG 1-X8 Resin

140-1441
500 g, analytical grade anion exchange resin, chloride form, 8% crosslinkage, 100–200 dry mesh size, 106–180 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #140-1451
AG 1-X8 Resin

140-1451
500 g, analytical grade anion exchange resin, chloride form, 8% crosslinkage, 200–400 dry mesh size, 45–106 µm wet bead size, ~1,000 MW limit

List Price:   $548.00
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AG 1-X2 Resin #140-1422
AG 1-X8 Resin

140-1422
500 g, analytical grade anion exchange resin, hydroxide form, 8% crosslinkage, 20–50 dry mesh size, 300–1,180 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #140-1443
AG 1-X8 Resin

140-1443
500 g, analytical grade anion exchange resin, acetate form, 8% crosslinkage, 100–200 dry mesh size, 106–180 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #140-1453
AG 1-X8 Resin

140-1453
500 g, analytical grade anion exchange resin, acetate form, 8% crosslinkage, 200–400 dry mesh size, 45–106 µm wet bead size, ~1,000 MW limit

List Price:   $558.00
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AG 1-X2 Resin #140-1444
AG 1-X8 Resin

140-1444
500 g, analytical grade anion exchange resin, formate form, 8% crosslinkage, 100–200 dry mesh size, 106–180 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #140-1454
AG 1-X8 Resin

140-1454
500 g, analytical grade anion exchange resin, formate form, 8% crosslinkage, 200–400 dry mesh size, 45–106 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #140-2421
AG 2-X8 Resin

140-2421
500 g, analytical grade anion exchange resin, chloride form, 8% crosslinkage, 20–50 dry mesh size, 300–1,180 µm wet bead size, ~1,000 MW limit

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AG 1-X2 Resin #143-1255
AG 1-X2 Resin

143-1255
100 g, biotechnology grade anion exchange resin, hydroxide form, 2% crosslinkage, 200–400 dry mesh size, 75–180 µm wet bead size, ~2,700 MW limit

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AG 1-X2 Resin #143-1345
AG 1-X4 Resin

143-1345
100 g, biotechnology grade anion exchange resin, hydroxide form, 4% crosslinkage, 100–200 dry mesh size, 63–150 µm wet bead size, ~1,400 MW limit

List Price:   $415.00
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AG 1-X8 Resin

143-2445
100 g, biotechnology grade anion exchange resin, hydroxide form, 8% crosslinkage, 100–200 dry mesh size, 106–180 µm wet bead size, ~1,000 MW limit

List Price:   $776.00
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AG 1-X8 Resin

143-2446
100 g, biotechnology grade anion exchange resin, hydroxide form, 8% crosslinkage, 200–400 dry mesh size, 45–106 µm wet bead size, ~1,000 MW limit

List Price:   $431.00
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Number Description Options
LIT212 Instruction Manual, AG 1, AG MP-1, and AG 2 Strong Anion Exchange Resin, Rev C Click to download