Affi-Prep Polymyxin Media

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Overview

Affinity Media Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi (3.1 bar) Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, provided charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi (6.8 bar) Purification of recombinant His-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi (3.1 bar) Purification of recombinant GST-tagged proteins
Profinity eXact*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free protein by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi (1 bar) Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG, per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi (1 bar) Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum protiens 0.14 ml serum/ml gel 2–10 15 psi (1 bar) Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum protiens 0.17–0.5 ml serum/ml gel 2–11 15 psi (1 bar) Produces albumin and protease-free antibody preparation from serum without prior dialysis
Affi-Prep polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi (70 bar) Endotoxin removal
Affi-Gel boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi (1 bar) Adsorption of cis-hydroxyl-containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi (5.5 bar) Activated matrix for the immobilization of barious ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi (1 bar) For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi (1 bar) For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi (1 bar) Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi (1 bar) Carbodimide coupling of carboxyl-containing ligand

* Refer to bulletin 3193 for purification conditions.
** For example, Profinity GST resin is only available in prepacked cartridges.
***Profinity eXact purification resin.

Affi-Prep polymyxin support consists of 2–4 mg of USP-grade polymyxin per ml of the macroporous polymeric Affi-Prep support. Affi-Prep polymyxin support binds endotoxins from a number of different strains of gram-negative bacteria, including Escherichia coli, Salmonella abortus, Salmonella minnesota, and Serratia marcescens.

Features of Affi-Prep polymyxin support include:

  • Endotoxin removal in research and process-scale applications
  • Linear flow rates to 2,000 cm/hr
  • Pressure stability to 70 bar or 1,000 psi
  • High chemical stability (withstands sanitization with 0.1 M NaOH)
Affi-Prep Polymyxin Support

156-0010
25 ml, ready-to-use affinity support, 2–4 mg USP-grade polymyxin per ml of macroporous support

List Price:   $786.00
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LIT280 Instruction Manual, Affi-Prep Polymyxin 25 ml, Rev B Click to download
1429 Affi-Prep Polymyxin Support Product Information Sheet, Rev A Click to download