Chromatography on Affi-Gel protein A media yields highly purified immunoglobulins (IgGs), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.
- High purity of IgGs
- High affinity for mammalian IgG
- High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
With Affi-Gel protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than is possible with standard methods. In addition, the MAPS process and Affi-Gel protein A media permit greater binding of mouse IgG1 than do immobilized protein G media.