Affi-Gel® Protein A Resin

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Affi-Gel Protein A Media

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Capacities of Protein A Supports
Immunoglobulin Affi-Gel Protein A
Capacity (mg/ml)
Affi-Prep Protein A
Capacity (mg/ml)
Mouse lgG1 6–10 7–9
Mouse lgG2a 6–10 7–9
Mouse lgG2b 6–10 7–9
Mouse lgG3 6–10 7–9
Mouse lgM* 6–10 7–9
Human lgG 15 10–12
Sheep lgG 8–10 7–9
Cow lgG 8–10 7–9
Horse lgG 8–10 7–9
Goat lgG 8–10 7–9
Rabbit lgG 8–10 7–9
Dog lgG 8–10 7–9
Pig lgG 8–10 7–9

* Approximately 50% of all mouse lgMs bind using the MAPS buffer system.

Technical Properties of UNOsphere SUPrA Affinity Media
Composition Highly Crosslinked Polyacrylamide Polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Dynamic Binding Capacity1 150 cm/hour, 30±3 mg/ml
300 cm/hour, 25±2 mg/ml
450 cm/hour, 20±2 mg/ml
Chemical Stability2 10 mM Hydrochloric Acid
6 M Guanidine Hydrochloride
0.1 M Arginine (pH 2.8)
0.1 M Citrate (pH 2.8)
0.1 M Glycine (pH 2.8)
Working pH Range 3–11
Cleaning-In-Place (CIP) 6 M Guanidine Hydrochloride
10 mM Hydrochloric Acid
0.1 M Sodium Hydroxide
1 M Acetic Acid/20% Ethanol
Recommended Mobile Phase Velocity Range 100–600 cm/hr
Temperature Stability 4–40°C
Delivery Conditions 50% slurry in 20% Ethanol
Storage Conditions 4–8°C

1 - Minimum 20 mg/ml at 300 cm/hr; 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column
2 - No significant change in chromatographic performance after 24 hour storage at room temperature.

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Chromatography on Affi-Gel protein A media yields highly purified immunoglobulins (IgGs), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

With Affi-Gel protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than is possible with standard methods. In addition, the MAPS process and Affi-Gel protein A media permit greater binding of mouse IgG1 than do immobilized protein G media.

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Affi-Gel Protein A Support

1536153
5 ml, ready-to-use protein A crosslinked agarose support

0 1536153 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6153_0921120656703.xml 1536153 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1536153-affi-gel-protein-support 1536153 Affi-Gel Protein A Support Affi-Gel Protein A Support 5 ml, ready-to-use protein A crosslinked agarose support 5 ml, ready-to-use protein A crosslinked agarose support 5 ml, ready-to-use protein A crosslinked agarose support /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6153_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6153_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6153_feature.jpg RepEx 153-6153 5 ml, ready-to-use protein A crosslinked agarose support 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536153&CartQuote=QT&quantity= 1251 false true false $357.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536153&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536153&quantity= 1536153
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Affi-Gel Protein A Support

1536154
50 ml, ready-to-use protein A crosslinked agarose support

1 1536154 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6154_0921120656745.xml 1536154 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1536154-affi-gel-protein-support 1536154 Affi-Gel Protein A Support Affi-Gel Protein A Support 50 ml, ready-to-use protein A crosslinked agarose support 50 ml, ready-to-use protein A crosslinked agarose support 50 ml, ready-to-use protein A crosslinked agarose support /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6154_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6154_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6154_feature.jpg RepEx 153-6154 50 ml, ready-to-use protein A crosslinked agarose support 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536154&CartQuote=QT&quantity= 1251 false true false $2,651.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536154&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536154&quantity= 1536154
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Affi-Gel Protein A MAPS II Kit

1536159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1

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Hazardous  
Protein A MAPS II Binding Buffer

1536161
Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L

3 1536161 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6161_0921120656724.xml 1536161 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6161_0921120656724.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jb6po1yn 2017-11-14 07:08:02 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:02 1536161-protein-maps-ii-binding-buffer 1536161 Protein A MAPS II Binding Buffer Protein A MAPS II Binding Buffer Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6161_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6161_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6161_feature.jpg RepEx 153-6161 Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536161&CartQuote=QT&quantity= 1272 false true false $886.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536161&AddToDefaultWishList=true&quantity= true false true 12161700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536161&quantity= 1536161
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Econo-Pac Protein A Columns

7322022
Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel

4 7322022 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml 7322022 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2022_0921120637443.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jb6po1z5 2017-11-14 07:07:55 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:55 7322022-econo-pac-protein-columns 7322022 Econo-Pac Protein A Columns Econo-Pac Protein A Columns Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-2022_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-2022_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-2022_feature.jpg RepEx 732-2022 Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Columns N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&CartQuote=QT&quantity= 7653 false true false $588.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322022&quantity= 7322022
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Econo-Pac® Protein A Kit

7322020
Purification kit, includes Affi-Gel® protein A column, four 10DG desalting columns, buffers

5 7322020 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml 7322020 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2020_0921120637502.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jb6po1zl 2017-11-14 07:07:55 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:55 7322020-econo-pac-protein-kit 7322020 Econo-Pac Protein A Kit Econo-Pac<sup>&reg;</sup> Protein A Kit Purification kit, includes Affi-Gel<sup>&reg;</sup> protein A column, four 10DG desalting columns, buffers Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel Purification kit, includes Affi-Gel<sup>&reg;</sup> protein A column, four 10DG desalting columns, buffers /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-2020_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-2020_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-2020_feature.jpg RepEx 732-2020 Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Columns N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&CartQuote=QT&quantity= 7653 true true false $350.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322020&quantity= 7322020
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Econo-Pac 10DG Desalting Columns

7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit

6 7322010 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2010_0921120637202.xml 7322010 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-2010_0921120637202.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jamoxzu7 2017-11-14 07:07:55 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:55 7322010-econo-pac-10dg-desalting-columns 7322010 Econo-Pac 10DG Desalting Columns Econo-Pac 10DG Desalting Columns Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-2010_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-2010_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-2010_feature.jpg RepEx 732-2010 Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Columns N 0 732-2010, 7322010, econo-pac, 10dg, desalting, columns, econopac, econo, pac, chromatography, protein, purification, buffer, exchange, gel, filtration, prepacked, gravity, flow, gravity-flow, column 7325314 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-5314_0921120619195.xml 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml 7880003 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/788-0003_1356736009.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322010&CartQuote=QT&quantity= 7653 false true false $210.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322010&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7322010&quantity= 7322010
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Poly Column Rack

7317005
Pkg of 1, 20-place rack, with removable tube rack, for use with Econo-Pac® or Poly-Prep® Chromatography Columns

7 7317005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-7005_0921120638974.xml 7317005 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-7005_0921120638974.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jb6po1yr 2017-11-14 07:07:55 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:55 7317005-poly-column-rack 7317005 Poly Column Rack Poly Column Rack Pkg of 1, 20-place rack, with removable tube rack, for use with <a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup></a> or <a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup></a> Chromatography Columns Pkg of 1, 20-place rack, with removal tube rack, for use with Econo-Pac chromatography columns (#731-1550, 731-1553) /webroot/web/images/ps/products/affinity_media/sku_thumb/global/731-7005_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/731-7005_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/731-7005_feature.jpg preview RepEx 731-7005 Pkg of 1, 20-place rack, with removable tube rack, for use with Econo-Pac or Poly-Prep Chromatography Columns 7317005, poly, column, rack, 20-tube, econo-pac, poly-prep, empty, plastic, chromatography, columns, accessories, protein, purification, separation, 7311550, 7311553, 7321010, 7321011 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Columns N 0 7311550 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-1550_0921120639297.xml 7311553 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-1553_0921120639335.xml 7880003 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/788-0003_1356736009.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7317005&CartQuote=QT&quantity= 1241 false true false $465.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7317005&AddToDefaultWishList=true&quantity= true false true 41116000 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7317005&quantity= 7317005
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Econo-Pac Flow Adaptor

7380019
Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns

8 7380019 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml 7380019 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po204jb6po205" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po204jb6po205"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/738-0019_0921120612326.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="16" DayOfWeek="6" Year="2017" Hour="0" Minute="48" Second="58" ZoneOffsetMillis="0" Time="1513385338476">Sat Dec 16 00:48:58 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jb6po1ym 2017-11-14 07:08:06 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:06 7380019-econo-pac-flow-adaptor 7380019 Econo-Pac Flow Adaptor Econo-Pac Flow Adaptor Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns Pkg of 1, 1.5 cm column ID, for use with Econo-Pac chromatography columns /webroot/web/images/ps/products/affinity_media/sku_thumb/global/738-0019_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/738-0019_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/738-0019_feature.jpg preview RepEx 738-0019 Pkg of 1, 1.5 cm column ID, for use with Econo-Column chromatography columns 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 <p>Use the Econo-Pac flow adaptor to improve column performance by eliminating head space above the gel bed and protecting it from disruption during sample loading.</p> /webroot/web/html/lsr/products/SKUs/autopopulated 738-0019.html /webroot/web/html/lsr/products/SKUs/autopopulated/738-0019.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&CartQuote=QT&quantity= 1241 false true false $149.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&AddToDefaultWishList=true&quantity= true false true 41115700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7380019&quantity= 7380019
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Number Description Options
5334 A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A Click to download
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