Affi-Gel Protein A Media

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Overview

Capacities of Protein A Supports
Immunoglobulin Affi-Gel® Protein A
Capacity (mg/ml)
Affi-Prep® Protein A
Capacity (mg/ml)
Mouse lgG1 6–10 7–9
Mouse lgG2a 6–10 7–9
Mouse lgG2b 6–10 7–9
Mouse lgG3 6–10 7–9
Mouse lgM* 6–10 7–9
Human lgG 15 10–12
Sheep lgG 8–10 7–9
Cow lgG 8–10 7–9
Horse lgG 8–10 7–9
Goat lgG 8–10 7–9
Rabbit lgG 8–10 7–9
Dog lgG 8–10 7–9
Pig lgG 8–10 7–9

* Approximately 50% of all mouse lgMs bind using the MAPS buffer system.

Technical Properties of UNOsphere SUPrA Affinity Media
Composition Highly Crosslinked Polyacrylamide Polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Dynamic Binding Capacity1 150 cm/hour, 30±3 mg/ml
300 cm/hour, 25±2 mg/ml
450 cm/hour, 20±2 mg/ml
Chemical Stability2 10 mM Hydrochloric Acid
6 M Guanidine Hydrochloride
0.1 M Arginine (pH 2.8)
0.1 M Citrate (pH 2.8)
0.1 M Glycine (pH 2.8)
Working pH Range 3–11
Cleaning-In-Place (CIP) 6 M Guanidine Hydrochloride
10 mM Hydrochloric Acid
0.1 M Sodium Hydroxide
1 M Acetic Acid/20% Ethanol
Recommended Mobile Phase Velocity Range 100–600 cm/hr
Temperature Stability 4–40°C
Delivery Conditions 50% slurry in 20% Ethanol
Storage Conditions 4–8°C

1 - Minimum 20 mg/ml at 300 cm/hr; 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column
2 - No significant change in chromatographic performance after 24 hour storage at room temperature.



Chromatography on Affi-Gel protein A media yields highly purified immunoglobulins (IgGs), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

With Affi-Gel protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than is possible with standard methods. In addition, the MAPS process and Affi-Gel protein A media permit greater binding of mouse IgG1 than do immobilized protein G media.

Affi-Gel Protein A Support

153-6153
5 ml, ready-to-use protein A crosslinked agarose support

List Price:   $307.00
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Affi-Gel Protein A Support

153-6154
50 ml, ready-to-use protein A crosslinked agarose support

List Price:   $2,304.00
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Affi-Gel Protein A MAPS II Kit

153-6159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1

List Price:   $442.00
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Hazardous  
Protein A MAPS II Binding Buffer

153-6161
Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L

List Price:   $784.00
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Number Description Options
5334 A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A Click to download
LIT88 Instruction Manual, Affi-Gel Protein A MAPS II Kit Click to download
LIT153 Instruction Manual, Econo-Pac Protein A Kit, Protein A Columns, Rev B Click to download [ Add to Cart (Free) ]