Affi-Gel® Hz Hydrazide Gel

Print
Affi-Gel Hz

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Scroll upScroll down


Affi-Gel Hz hydrazide gel activated support couples immunoglobulin G (IgG) molecules via carbohydrate moieties on the Fc region of antibody molecules. Fc attachment results in a more specific antigen-antibody interaction and 100–300% higher antigen binding capacity than other resins.

Affi-Gel Hz support advantages include:

  • Stable covalent hydrazone bonds
  • Mild oxidation without alteration of antibody activity
  • High antigen binding capacity
  • pH stability
x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Affi-Gel Hz Hydrazide Gel

1536047
25 ml, hydrazide gel activated support

0 1536047 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6047_0921120657928.xml 1536047 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1536047-affi-gel-hz-hydrazide-gel 1536047 Affi-Gel Hz Hydrazide Gel Affi-Gel Hz Hydrazide Gel 25 ml, hydrazide gel activated support 25 ml, hydrazide gel activated support 25 ml, hydrazide gel activated support /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-6047_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-6047_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-6047_feature.jpg RepEx 153-6047 25 ml, hydrazide gel activated support 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536047&CartQuote=QT&quantity= true true false $341.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536047&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536047&quantity= 1536047
List Price: 
 
$341.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Hazardous  
Affi-Gel Hz Immunoaffinity Kit

1536060
Hydrazide-activated affinity media kit, includes 5 m Affi-Gel Hz gel, 2 x 25 mg oxidizer, and 25 ml coupling buffer concentrate, Econo-Pac 10DG desalting columns, Econo-Column column

1 1536060 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6060_0921120657906.xml 1536060 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1536060-affi-gel-hz-immunoaffinity-kit 1536060 Affi-Gel Hz Immunoaffinity Kit Affi-Gel Hz Immunoaffinity Kit Hydrazide-activated affinity media kit, includes 5 m Affi-Gel Hz gel, 2 x 25 mg oxidizer, and 25 ml coupling buffer concentrate, Econo-Pac 10DG desalting columns, Econo-Column column Hydrazide-activated affinity media kit, includes 5 m Affi-Gel Hz gel, 2 x 25 mg oxidizer, and 25 ml coupling buffer concentrate, Econo-Pac 10DG desalting columns, Econo-Column column Hydrazide-activated affinity media kit, includes 5 m Affi-Gel Hz gel, 2 x 25 mg oxidizer, and 25 ml coupling buffer concentrate, Econo-Pac 10DG desalting columns, Econo-Column column /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6060_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6060_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6060_feature.jpg RepEx 153-6060 Hydrazide-activated affinity media kit, includes 5 m Affi-Gel Hz gel, 2 x 25 mg oxidizer, and 25 ml coupling buffer concentrate, Econo-Pac 10DG desalting columns, Econo-Column column 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536060&CartQuote=QT&quantity= true true false $350.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536060&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536060&quantity= 1536060
List Price: 
 
$350.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Hazardous  
Affi-Gel Hz 10x Coupling Buffer Concentrate

1536054
500 ml, coupling buffer concentrate for use with Affi-Gel Hz hydrazide gel

2 1536054 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6054_0921120657971.xml 1536054 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1536054-affi-gel-hz-10x-coupling-buffer-concentrate 1536054 Affi-Gel Hz 10x Coupling Buffer Concentrate Affi-Gel Hz 10x Coupling Buffer Concentrate 500 ml, coupling buffer concentrate for use with Affi-Gel Hz hydrazide gel 500 ml, coupling buffer concentrate for use with Affi-Gel Hz hydrazide gel 500 ml, coupling buffer concentrate for use with Affi-Gel Hz hydrazide gel /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-6054_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-6054_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-6054_feature.jpg RepEx 153-6054 500 ml, coupling buffer concentrate for use with Affi-Gel Hz hydrazide gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536054&CartQuote=QT&quantity= false true false $160.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536054&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536054&quantity= 1536054
List Price: 
 
$160.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
Affi-Gel Oxidizer

1536055
250 mg, sodium periodate; for use with Affi-Gel Hz gel or Affi-Prep Hz support

3 1536055 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6055_0921120657949.xml 1536055 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:02 1536055-affi-gel-oxidizer 1536055 Affi-Gel Oxidizer Affi-Gel Oxidizer 250 mg, sodium periodate; for use with Affi-Gel Hz gel or Affi-Prep Hz support 250 mg, sodium periodate; for use with Affi-Gel Hz gel or Affi-Prep Hz support 250 mg, sodium periodate; for use with Affi-Gel Hz gel or Affi-Prep Hz support /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6055_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6055_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6055_feature.jpg RepEx 153-6055 250 mg, sodium periodate; for use with Affi-Gel Hz gel or Affi-Prep Hz support 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536055&CartQuote=QT&quantity= true true false $63.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536055&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536055&quantity= 1536055
List Price: 
 
$63.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Hazardous  
Number Description Options
LIT101 Instruction Manual, Affi-Gel Hz Immunoaffinity Kit, Rev C Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.