Affi-Gel® Blue Gel

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Affi-Gel Blue Gel

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Structure of Affi-Gel Blue Gel
Structure of Affli-Gel blue gel
Purification Applications of Blood Proteins on Affi-Gel Blue Gel

Affi-Gel blue gel has been used to separate and purify a number of different serum and plasma proteins:

  • Purification of human serum complement
  • Purification of rat alpha fetoprotein
  • Purification of rat serum albumin
  • Separation and purification of plasma proteins
  • Purification of alpha-2 macroglobulin
  • Characterization of fragments of human albumin
  • Albumin removal from serum
  • Isolation of sarcoplasmic reticulum vesicles
  • Purification of equine thyromedin
  • Affinity separation of human plasma gelsolin
To read more click here
Affi-Gel Blue Gel Enzyme Applications
Enzyme Source Eluant
3',5' cyclic AMP phosphodiesterase Dictyostelium discoideum 0.18 M NaCl
3'-PGA diesterase Yeast 0.6 M KCl in 50 mM Tris-HCl
5-methyl-L-tetrahydrofolate reductase Bovine liver 0.4–3.0 M KCl
Acylation stimulating protein Human plasma 3 M NaCl, 0.02 M phosphate
Adenylate cyclase Bovine brain 1 mM KCl, 8 mM ATP,
16 mM MgCl2, 1 mM EDTA
Alkaline phosphatase E. coli
Alkyl hydroperoxide reductase S. typhimurium 1.0 M KCl, 0.5 M NaCl
Aspergillins (RIPs) Aspergillus 0.5–1 M NaCl gradient
ATP:AMP phosphotransferase Bovine heart 0.4–2.0 M NaCl
Calmodulin-dependent cyclic
nucleotide phosphodiesterase
Bovine brain 0.15–1.5 M NaCl
Candida acid proteinase (CAP) C. albicans 1.5 M NaCl in citrate buffer
Carbamyl phosphate synthetase Frog liver 1 mM dithiothreitol
Casein kinase I Bovine sperm 0.2–2.5 M NaCl gradient
DNA helicase S. cerevisiae 1 M NaCl
DNA polymerase Calf thymus 0–0.5 M KCl
Formamidopyrimidine-DNA glycosylase E. coli 0.1–0.8 M KCl
GAL4(63) S. cerevisiae STD, 250 mM NaCl
Glutamate dehydrogenase Yeast 10 mM NADH, 1 M NH4Cl
Glutamine synthetase S. typhimurium 5 mM ATP
Glyoxalase II Rat erythrocytes 0–0.2 M KCl
GMP reductase Human erythrocytes 1 mM NADPH, 2 mM GMP
GTP:RNA guanylytransferase Wheat germ 0.05–0.75 M NaCl
Isocitrate dehydrogenase E. coli 2 mM NADP
Isocitrate dehydrogenase E. coli 2 mM NADP+
MB creatine kinase Human heart 0.25 M NaCl
Membrane-bound phosphatidylinositol
kinase
Rat brain Triton X-100, NaCl, glycerol
Methylenetetrahydrofolate reductase Porcine liver 0–10 mM NADPH
Metmyoglobin reductase Bovine heart 1.0 M NaCl, 1 mM NADH+
Mitochondrial hexokinase Rat brain Glucose 6-phosphate
Phosphodiesterase Bovine brain 0.2 M KCl
Phosphatidic acid phosphatase Porcine thymus membranes 0.25–3 M NaCl gradient
Phosphoinositiede-specific
phospholipase C (PIC)
Bovine heart 2 M NaCl
Polyadenylate-binding protein (PABP) P. sativum 2 M Guanidine-HCl
RNA ligase E. coli 0.2 M NaCl, 2 mM ATP
Serine transhydroxymethylase Porcine liver 0.5 M KCl
Thymidylate synthetase S. cervisiae 0.25–1 M KCl
Tyrosine phenollyase Erwinia herbicola 1.5 M NaCl,
0.5 mM mercaptoethanol

To read more click here

Removal of Albumin

Comparison of Cibacron Blue Affinity-Based Formats for Depletion of Albumin From Samples Prior to Two-Dimensional Gel Electrophoresis

Test Sample Spot Number % Change % Albumin Depletion
Control 670 N/A N/A
Batch Binding 620 -6% 45%
Gel loader columns 650 -3% 36%
Aurum serum protein mini kit 700 +4% >80%
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Affi-Gel blue affinity gel is a beaded, crosslinked agarose gel with covalently attached Cibacron Blue F3GA dye. It contains >1.9 mg dye per ml of gel and has a typical albumin capacity of >11 mg/ml. Affi-Gel Blue gel purifies a large range of proteins from widely divergent origins. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site in various applications.

Affi-Gel Blue is ideally suited for albumin removal (using 50–100 mesh) and enzyme purification (using 100–200 mesh). Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of the eluent buffers.

For more information, request bulletin 1107.

Functional group Cibacron blue
Ligand concentration ≥ 1.9 mg/ml
Typical protein capacity > 11 mg/ml
pH stability 4–11
Pressure limit 15 psi
Flow rate 15–25 cm/hr
Bead size:
   50–100 mesh
   100–200 mesh

150–300 µm
75–150 µm
Storage 4°C
Shipping buffer 0.05 % NaN3 in water
Sanitization 0.1 M NaOH
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Affi-Gel Blue Gel

1537301
100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye

2 1537301 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7301_0921120658700.xml 1537301 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldlq 2017-11-14 07:08:02 true true internet/product_SKU jthvldlq templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7301_0921120658700.xml LSR 2019-05-04 20:55:54 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998735900 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:54 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7301_0921120658700.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7301_0921120658700.xml 1537301-affi-gel-blue-gel 1537301 Affi-Gel Blue Gel Affi-Gel Blue Gel 100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-7301_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-7301_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-7301_feature.jpg RepEx 153-7301 100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 false $611.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537301&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537301&CartQuote=QT&quantity= 1251 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537301&AddToDefaultWishList=true&quantity= 41115700 611.00 1537301
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Affi-Gel Blue Gel

1537302
100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye

3 1537302 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7302_0921120658719.xml 1537302 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldpf 2017-11-14 07:08:02 true true internet/product_SKU jthvldpf templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7302_0921120658719.xml LSR 2019-05-04 20:56:05 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998735942 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7302_0921120658719.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-7302_0921120658719.xml 1537302-affi-gel-blue-gel 1537302 Affi-Gel Blue Gel Affi-Gel Blue Gel 100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-7302_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-7302_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-7302_feature.jpg RepEx 153-7302 100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 false $611.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537302&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537302&CartQuote=QT&quantity= 1251 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1537302&AddToDefaultWishList=true&quantity= 23151800 611.00 1537302
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Bio-Scale Mini Affinity Sampler Pack

7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue

4 7324651 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml 7324651 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0tenn 2017-11-14 07:08:06 true true internet/product_SKU jao0tenn templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml LSR 2019-05-04 20:56:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998747568 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4651_0921120612199.xml 7324651-bio-scale-mini-affinity-sampler-pack 7324651 Bio-Scale Mini Affinity Sampler Pack Bio-Scale Mini Affinity Sampler Pack Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-4651_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-4651_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-4651_feature.jpg RepEx 732-4651 Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false $735.00 false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324651&AddToDefaultWishList=true&quantity= 41116100 735.00 7324651
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Bio-Scale Mini Affi-Gel Blue Cartridge

7324642
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

5 7324642 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4642_0921120647883.xml 7324642 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldpx 2017-11-14 07:07:56 true true internet/product_SKU jthvldpx templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4642_0921120647883.xml LSR 2019-05-04 20:56:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997273036 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4642_0921120647883.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4642_0921120647883.xml 7324642-bio-scale-mini-affi-gel-blue-cartridge 7324642 Bio-Scale Mini Affi-Gel Blue Cartridge Bio-Scale Mini Affi-Gel Blue Cartridge Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-4642_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-4642_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-4642_feature.jpg preview ,VN RepEx 732-4642 Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system 7324642, bio-scale, mini, affi-gel, blue, cartridge, affigel, prepacked, affinity, column, biologic, chromatography, protein, purification 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $160.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324642&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324642&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324642&AddToDefaultWishList=true&quantity= 23151800 160.00 7324642
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Bio-Scale Mini Affi-Gel Blue Cartridges

7324644
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

6 7324644 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4644_0921120647902.xml 7324644 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldih 2017-11-14 07:07:56 true true internet/product_SKU jthvldih templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4644_0921120647902.xml LSR 2019-05-04 20:55:44 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997273078 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:44 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4644_0921120647902.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4644_0921120647902.xml 7324644-bio-scale-mini-affi-gel-blue-cartridges 7324644 Bio-Scale Mini Affi-Gel Blue Cartridges Bio-Scale Mini Affi-Gel Blue Cartridges Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4644_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4644_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4644_feature.jpg preview ,VN RepEx 732-4644 Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system 7324644, bio-scale, mini, affi-gel, blue, cartridge, affigel, prepacked, affinity, column, biologic, chromatography, protein, purification 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $546.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324644&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324644&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324644&AddToDefaultWishList=true&quantity= 23151800 546.00 7324644
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Aurum Affi-Gel Blue Mini Kit

7326712
Kit for removal of albumin from serum/plasma samples, includes Micro Bio-Spin columns prepacked with Affi-Gel Blue gel for 2 preps

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Aurum Affi-Gel Blue Mini Columns (7326708)
Aurum Affi-Gel Blue Mini Columns

7326708
Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples

8 7326708 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Depletion-/732-6708_0921120738117.xml 7326708 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jthvlgue 2017-11-14 07:09:00 true true internet/product_SKU jthvlgue templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Depletion-/732-6708_0921120738117.xml LSR 2019-05-04 16:23:47 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556043812664 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:47 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Depletion-/732-6708_0921120738117.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Depletion-/732-6708_0921120738117.xml 7326708-aurum-affi-gel-blue-mini-columns 7326708 Aurum Affi-Gel Blue Mini Columns Aurum Affi-Gel Blue Mini Columns Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples /webroot/web/images/ps/products/affinity_media/sku_thumb/global/732-6708_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/732-6708_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/732-6708_feature.jpg RepEx 732-6708 Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Protein-Sample-Depletion- N 0 false $430.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326708&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326708&CartQuote=QT&quantity= 1453 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326708&AddToDefaultWishList=true&quantity= 41115700 430.00 7326708
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Number Description Options
1107 Affi-Gel® Blue Affinity Chromatography Gel for Enzyme and Blood Protein Purifications Click to download
4110139 Instruction Manual, Aurum™ Affi-Gel Blue Mini Kits and Columns Sample Preparation, Rev B Click to download
LIT590 Instruction Manual, Affi-Gel Blue Gel Click to download
3168 Comparison of Cibacron Blue Affinity-Based Formats for Depletion of Albumin From Samples Prior to Two-Dimensional Gel Electrophoresis Click to download

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4d1854d4-ce76-4696-b63c-68bd767d02a8 [x-forwarded-proto] = [http] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [80] [x-forwarded-for] = [34.239.158.107, 10.232.2.84] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/product/affi-gel-blue-gel] [x-amzn-trace-id] = [Root=1-5d59ac21-eda544541fb8b411fe335aa3] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=4d1854d4-ce76-4696-b63c-68bd767d02a8] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/product/affi-gel-blue-gel] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductDetail pageStyleKey affi-gel-blue-gel PSD https://www.bio-rad-antibodies.com https://www.bio-rad.com 03/26/14 02:17 PM en_US true internet/generic 2019-07-09 16:59:23 /default/main/bio-rad/STAGING 1385504243813 US 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