Affi-Gel® and Affi-Prep® Protein A Media

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Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.

With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.

Affi-Gel Protein A Support

153-6153
5 ml, ready-to-use protein A crosslinked agarose support

List Price:   $307.00
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Affi-Gel Protein A Support

153-6154
50 ml, ready-to-use protein A crosslinked agarose support

List Price:   $2,304.00
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Affi-Gel Protein A MAPS II Kit

153-6159
Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1

List Price:   $442.00
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Hazardous  
Affi-Prep Protein A Support

156-0006
5 ml, ready-to-use pressure-stable polymer support

List Price:   $389.00
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Affi-Prep Protein A Support

156-0005
25 ml, ready-to-use pressure-stable polymer support

List Price:   $1,848.00
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Protein A MAPS II Binding Buffer

153-6161
Optimized buffer concentrate, for use with Affi-Gel and Affi-Prep protein A media, makes 5 L

List Price:   $784.00
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Number Description Options
LIT153 Instruction Manual, Econo-Pac Protein A Kit, Protein A Columns, Rev B Click to download [ Add to Cart (Free) ]
LIT88 Instruction Manual, Affi-Gel Protein A MAPS II Kit Click to download
LIT79 Instruction Manual, Affi-Prep Protein A MAPS II Kit Click to download [ Add to Cart (Free) ]
LIT230 Instruction Manual, Affi-Prep Protein A Matrix Click to download [ Add to Cart (Free) ]
LIT379 Affi-Gel Protein A Agarose for IgG Purification Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]