ReadyPrep™ 2-D Cleanup Kit

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Overview

SDS Removal

Before

After


Detergent removal using the ReadyPrep™ 2-D cleanup kit. E. coli extracts containing 1% SDS were electrophorised before and after treatment with the ReadyPrep 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip™ pH 3–10 IPG strips, then run on Criterion™ 8–16 Tris-HCl precast gels for the second dimension.

Salt Removal

Salt removal using the ReadyPrep™ 2-D cleanup kit. E. coli extracts containing 1 M NaCl were electrophorised before and after treatment with the ReadyPrep 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip™ pH 3–10 IPG strips, then run on Criterion™ 8–16 Tris-HCl precast gels for the second dimension.

2-D Video Tutorial


The ReadyPrep 2-D cleanup kit uses selective precipitation to remove ionic contaminants such as detergents, lipids, and phenolic compounds from protein samples. These contaminants may interfere with separation, particularly during IEF and 2-D separations. Use of the kit improves 2-D resolution and reproducibility. This kit can also be used to clean up samples prior to other applications in which contaminants may negatively impact results.

The ReadyPrep 2-D cleanup kit:

  • Employs a TCA-like protein precipitation to remove ionic contaminants from protein samples
  • Enables concentration of proteins from samples that are too dilute, allowing for higher protein loads that can improve spot detection
  • Can be used with virtually any protein sample
  • Reduces streaking, background staining, and other gel artifacts associated with ionic contaminants
  • Provides reagents for either 5 or 50 preparations

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ReadyPrep™ 2-D Cleanup Kit

1632130
50 preps, general purpose protein preparation kit, includes precipitation agent unaffected by detergents, chaotropic agents, and other common reagents

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$305.00
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ReadyPrep™ 2-D Cleanup Kit

1632140
5 preps, general purpose protein preparation kit, includes precipitation agent unaffected by detergents, chaotropic agents, and other common reagents

List Price:
 
$120.00
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Number Description Options
4110143 Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A Click to download [ Add to Cart (Free) ]
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Rev B Click to download [ Add to Cart (Free) ]