PrimePCR™ Template for SYBR® Green Assay: TBP, Human

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PrimePCR Primer Assays for Real-Time PCR synthetic positive control DNA template tube

Gene-specific template designed to give a positive real-time PCR result when used with the corresponding gene assay.

This template is intended for use with the real-time PCR SYBR Green assay with the following Unique Assay ID: qHsaCID0007122

Reaction:  200 x 20 µl reactions desalted
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Gene Information

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) which binds to the core promoter to position the polymerase properly serves as the scaffold for assembly of the remainder of the transcription complex and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription serve as coactivators function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39 and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17 a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2010]

Gene Symbol:   TBP
Gene Name:   TATA box binding protein
Aliases:   GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID
Ensembl:   ENSG00000112592
Entrez:   6908
UniGene:   Hs.590872
RefSeq:   NC_000006.11 NT_025741.15 NG_008165.1
Chromosome Mapping   6q27

Number Description Download
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev D Click to download
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