Gene:  ZP3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence a conserved ZP domain a C-terminal consensus furin cleavage site and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3 including the above described variation and maps closely to this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ZP3, Human
zona pellucida glycoprotein 3 (sperm receptor)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010073
Info:   Same primer pair as used in probe assay qHsaCIP0027441
List Price:    $125.00
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PrimePCR™ Probe Assay: ZP3, Human
zona pellucida glycoprotein 3 (sperm receptor)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027441
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ZP3, Human
zona pellucida glycoprotein 3 (sperm receptor)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016817
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010073; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ZP3, Human
zona pellucida glycoprotein 3 (sperm receptor)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048964
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027441; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ZP3, Human
zona pellucida glycoprotein 3 (sperm receptor)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048965
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027441; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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