Gene:  WISP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain von Willebrand factor type C module thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq Mar 2011]

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PrimePCR™ SYBR® Green Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036389
Info:   Same primer pair as used in probe assay qHsaCEP0039113
List Price:    $125.00
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PrimePCR™ Probe Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039113
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005664
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0036389; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000063
Info:   FAM: 85nt amplicon; intronic
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500487
Info:   FAM; 69nt amplicon; exonic; FAM version of WISP1 (dHsaCP2506641)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506641
Info:   HEX; 69nt amplicon; exonic; HEX version of WISP1 (dHsaCP2500487)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2507213
Info:   HEX; 85nt amplicon; intronic; HEX version of WISP1 (dHsaCP1000063)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029640
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039113; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WISP1, Human
WNT1 inducible signaling pathway protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029641
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039113; exonic
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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