Gene:  WFS1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a transmembrane protein which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain pancreas heart and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome also called DIDMOAD (Diabetes Insipidus Diabetes Mellitus Optic Atrophy and Deafness) an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6) also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2009]

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PrimePCR™ SYBR® Green Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003817
Info:   Recommended - best coverage
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043278
Info:   Same primer pair as used in probe assay qHsaCEP0050540
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050540
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5002190
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0003817; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008064
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043278; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034440
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050540; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WFS1, Human
Wolfram syndrome 1 (wolframin)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034441
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050540; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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