Gene:  VPS13A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene may control steps in the cycling of proteins through the trans-Golgi network to endosomes lysosomes and the plasma membrane. Mutations in this gene cause the autosomal recessive disorder chorea-acanthocytosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048259
Info:   Same primer pair as used in probe assay qHsaCEP0055519
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PrimePCR™ SYBR® Green Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014722
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055519
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012508
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048259; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020270
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014722; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043326
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055519; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: VPS13A, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043327
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055519; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:VPS13A p.R1268*, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504326
Info:   94nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:VPS13A WT for p.R1268*, Human
vacuolar protein sorting 13 homolog A (S. cerevisiae)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504327
Info:   94nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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