Gene:  VIM1, Arabidopsis

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Encodes a 645-amino acid methylcytosine-binding protein with a PHD domain, two RING finger domains, and an SRA domain that is involved in centromere heterochromatinization. This protein functions as an E3 ubiquitin ligase in vitro. The protein has been shown to bind to methylated cytosines of CG, CNG and CNN motifs via its SRA domain but has a preference for the former. It plays a role in the establishment/maintenance of chromatin structure during cell division and is localized in the nucleus. Plants over-expressing VIM1/ORTH2 show an inhibition in root growth and a delay in flowering. Both over-expression of GFP:ORTH2 and loss of ORTH2/VIM1 lead to decreased levels of DNA methylation. GFP:ORTH2 over-expressers also have increased levels of FWA transcripts.

PrimePCR™ SYBR® Green Assay: VIM1, Arabidopsis
Zinc finger (C3HC4-type RING finger) family protein

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qAthCED0050938
List Price:    $98.88
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PrimePCR™ Probe Assay: VIM1, Arabidopsis
Zinc finger (C3HC4-type RING finger) family protein

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qAthCEP0023780
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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