Gene:  USP18, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon suggesting a function of this protein in downregulating interferon responses independent of its isopeptidase activity towards ISG15. [provided by RefSeq Sep 2011]

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PrimePCR™ SYBR® Green Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003167
Info:   Recommended - best efficiency
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ SYBR® Green Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047706
Info:   Same primer pair as used in probe assay qHsaCEP0054966
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054966
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001692
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0003167; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012013
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047706; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042336
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054966; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: USP18, Human
ubiquitin specific peptidase 18

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042337
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054966; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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