Gene:  UPF3B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: UPF3B, Human
UPF3 regulator of nonsense transcripts homolog B (yeast)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011105
Info:   Same primer pair as used in probe assay qHsaCIP0027863
List Price:    $125.00
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PrimePCR™ Probe Assay: UPF3B, Human
UPF3 regulator of nonsense transcripts homolog B (yeast)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027863
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: UPF3B, Human
UPF3 regulator of nonsense transcripts homolog B (yeast)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5017632
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011105; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: UPF3B, Human
UPF3 regulator of nonsense transcripts homolog B (yeast)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5049716
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027863; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: UPF3B, Human
UPF3 regulator of nonsense transcripts homolog B (yeast)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5049717
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027863; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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