Gene:  UBR5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a progestin-induced protein which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057016
Info:   Same primer pair as used in probe assay qHsaCEP0058121
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PrimePCR™ SYBR® Green Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014740
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PrimePCR™ Probe Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058121
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013599
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0057016; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020284
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014740; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045508
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0058121; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: UBR5, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045509
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0058121; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:UBR5 p.R23G, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503480
Info:   77nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:UBR5 WT for p.R23G, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503481
Info:   77nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:UBR5 p.R1978*, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504890
Info:   96nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:UBR5 WT for p.R1978*, Human
ubiquitin protein ligase E3 component n-recognin 5

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504891
Info:   96nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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