Gene:  TTN, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions a N-terminal I-band and a C-terminal A-band. The I-band which is the elastic part of the molecule contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline glutamate valine and lysine. The A-band which is thought to act as a protein-ruler contains a mixture of immunoglobulin and fibronectin repeats and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and therefore to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq Feb 2012]

PrimePCR™ SYBR® Green Assay: TTN, Human
titin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020293
Info:   Same primer pair as used in probe assay qHsaCIP0032710
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PrimePCR™ Probe Assay: TTN, Human
titin

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0032710
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    Related pathways not available
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LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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