Gene:  TOP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: TOP1, Human
topoisomerase (DNA) I

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015613
Info:   Same primer pair as used in probe assay qHsaCIP0030049
List Price:    $130.00
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PrimePCR™ Probe Assay: TOP1, Human
topoisomerase (DNA) I

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030049
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TOP1, Human
topoisomerase (DNA) I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020958
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015613; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TOP1, Human
topoisomerase (DNA) I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053428
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030049; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TOP1, Human
topoisomerase (DNA) I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053429
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030049; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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