Gene:  TNNT3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which ultimately produces a muscle contraction. The troponin complex has protein subunits C I and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal- slow skeletal- and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq Oct 2009]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005032
Info:   Recommended - best coverage
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044325
Info:   Same primer pair as used in probe assay qHsaCEP0051587
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051587
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008991
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044325; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188055
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005032; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036294
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051587; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNT3, Human
troponin T type 3 (skeletal, fast)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036295
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051587; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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