Gene:  TNNI3K, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues such as liver and kidney and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream TNNI3 interacting kinase (TNNI3K) gene. [provided by RefSeq Dec 2010]

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PrimePCR™ SYBR® Green Assay: TNNI3K, Human
TNNI3 interacting kinase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038836
Info:   Same primer pair as used in probe assay qHsaCEP0041558
List Price:    $125.00
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PrimePCR™ Probe Assay: TNNI3K, Human
TNNI3 interacting kinase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0041558
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TNNI3K, Human
TNNI3 interacting kinase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188989
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0038836; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNI3K, Human
TNNI3 interacting kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5191669
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0041558; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNI3K, Human
TNNI3 interacting kinase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5191670
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0041558; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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